| pubmed-article:16183024 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C0034845 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C0597717 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C0599896 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:16183024 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:16183024 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:16183024 | pubmed:dateCreated | 2005-9-26 | lld:pubmed |
| pubmed-article:16183024 | pubmed:abstractText | Fluorescence imaging of living cells depends on an efficient and specific method for labeling the target cellular protein with fluorophores. Here we show that Sfp phosphopantetheinyl transferase-catalyzed protein labeling is suitable for fluorescence imaging of membrane proteins that spend at least part of their membrane trafficking cycle at the cell surface. In this study, transferrin receptor 1 (TfR1) was fused to peptide carrier protein (PCP), and the TfR1-PCP fusion protein was specifically labeled with fluorophore Alexa 488 by Sfp. The trafficking of transferrin-TfR1-PCP complex during the process of transferrin-mediated iron uptake was imaged by fluorescence resonance energy transfer between the fluorescently labeled transferrin ligand and TfR1 receptor. We thus demonstrated that Sfp-catalyzed small molecule labeling of the PCP tag represents a practical and efficient tool for molecular imaging studies in living cells. | lld:pubmed |
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| pubmed-article:16183024 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16183024 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16183024 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16183024 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16183024 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16183024 | pubmed:issn | 1074-5521 | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:WalshChristop... | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:GolanDavid... | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:Wessling-Resn... | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:YinJunJ | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:LinAlison JAJ | lld:pubmed |
| pubmed-article:16183024 | pubmed:author | pubmed-author:BuckettPeter... | lld:pubmed |
| pubmed-article:16183024 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16183024 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:16183024 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16183024 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16183024 | pubmed:pagination | 999-1006 | lld:pubmed |
| pubmed-article:16183024 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:meshHeading | pubmed-meshheading:16183024... | lld:pubmed |
| pubmed-article:16183024 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16183024 | pubmed:articleTitle | Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling. | lld:pubmed |
| pubmed-article:16183024 | pubmed:affiliation | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA. | lld:pubmed |
| pubmed-article:16183024 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16183024 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16183024 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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