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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-9-26
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pubmed:abstractText |
Fluorescence imaging of living cells depends on an efficient and specific method for labeling the target cellular protein with fluorophores. Here we show that Sfp phosphopantetheinyl transferase-catalyzed protein labeling is suitable for fluorescence imaging of membrane proteins that spend at least part of their membrane trafficking cycle at the cell surface. In this study, transferrin receptor 1 (TfR1) was fused to peptide carrier protein (PCP), and the TfR1-PCP fusion protein was specifically labeled with fluorophore Alexa 488 by Sfp. The trafficking of transferrin-TfR1-PCP complex during the process of transferrin-mediated iron uptake was imaged by fluorescence resonance energy transfer between the fluorescently labeled transferrin ligand and TfR1 receptor. We thus demonstrated that Sfp-catalyzed small molecule labeling of the PCP tag represents a practical and efficient tool for molecular imaging studies in living cells.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/GM20011,
http://linkedlifedata.com/resource/pubmed/grant/HL070819,
http://linkedlifedata.com/resource/pubmed/grant/HL32854,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056160-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056160-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056160-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056160-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056160-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK064750-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK064750-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK064750-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK064750-04
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-10075643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-10531064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-10940329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-11680618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-12469133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-12677058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-14529282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-14595367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-14980223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15034592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15038033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15123281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15212504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15264811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15317665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15379631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15493886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15681376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-15782206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-3611186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-6300904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-9325434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-9657724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-9759496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16183024-9782058
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1074-5521
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
999-1006
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16183024-Bacterial Proteins,
pubmed-meshheading:16183024-Blotting, Western,
pubmed-meshheading:16183024-Catalysis,
pubmed-meshheading:16183024-Cell Line,
pubmed-meshheading:16183024-Cloning, Molecular,
pubmed-meshheading:16183024-Endocytosis,
pubmed-meshheading:16183024-Fluorescence Resonance Energy Transfer,
pubmed-meshheading:16183024-Protein Transport,
pubmed-meshheading:16183024-Receptors, Transferrin,
pubmed-meshheading:16183024-Transferases (Other Substituted Phosphate Groups),
pubmed-meshheading:16183024-Transferrin
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pubmed:year |
2005
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pubmed:articleTitle |
Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling.
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pubmed:affiliation |
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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