Source:http://linkedlifedata.com/resource/pubmed/id/16179351
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
47
|
| pubmed:dateCreated |
2005-11-21
|
| pubmed:abstractText |
Superoxide dismutases (SODs) are important antioxidant enzymes responsible for the elimination of superoxide radical (O(2)(-)). The manganese-containing SOD (Mn-SOD) has been suggested to have tumor suppressor function and is located in the mitochondria where the majority of O(2)(-) is generated during respiration. Although increased reactive oxygen species (ROS) in cancer cells has long been recognized, the expression of Mn-SOD in cancer and its role in cancer development remain elusive. The present study used a human tissue microarray to analyze Mn-SOD expression in primary ovarian cancer tissues, benign ovarian lesions, and normal ovary epithelium. Significantly higher levels of Mn-SOD protein expression were detected in the malignant tissues compared with normal tissues (p < 0.05). In experimental systems, suppression of Mn-SOD expression by small interfering RNA caused a 70% increase of superoxide in ovarian cancer cells, leading to stimulation of cell proliferation in vitro and more aggressive tumor growth in vivo. Furthermore, stimulation of mitochondrial O(2)(-) production induced an increase of Mn-SOD expression. Our findings suggest that the increase in Mn-SOD expression in ovarian cancer is a cellular response to intrinsic ROS stress and that scavenging of superoxide by SOD may alleviate the ROS stress and thus reduce the simulating effect of ROS on cell growth.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
280
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
39485-92
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:16179351-Animals,
pubmed-meshheading:16179351-Base Sequence,
pubmed-meshheading:16179351-Cell Line, Tumor,
pubmed-meshheading:16179351-Cell Proliferation,
pubmed-meshheading:16179351-Cystadenoma,
pubmed-meshheading:16179351-Female,
pubmed-meshheading:16179351-Gene Expression Profiling,
pubmed-meshheading:16179351-Humans,
pubmed-meshheading:16179351-Mice,
pubmed-meshheading:16179351-Mitochondria,
pubmed-meshheading:16179351-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16179351-Ovarian Neoplasms,
pubmed-meshheading:16179351-Ovary,
pubmed-meshheading:16179351-Oxidative Stress,
pubmed-meshheading:16179351-RNA, Messenger,
pubmed-meshheading:16179351-RNA, Neoplasm,
pubmed-meshheading:16179351-RNA, Small Interfering,
pubmed-meshheading:16179351-Superoxide Dismutase,
pubmed-meshheading:16179351-Transfection
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Mitochondrial manganese-superoxide dismutase expression in ovarian cancer: role in cell proliferation and response to oxidative stress.
|
| pubmed:affiliation |
Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|