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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-11-2
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pubmed:abstractText |
We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT(1). All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT(1), but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT(1) expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-octadecyl)benzoylacrylic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Acrylates,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoates,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/L 663536,
http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene B4,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leukotriene B4,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Thiophenes,
http://linkedlifedata.com/resource/pubmed/chemical/methyl arachidonylfluorophosphonate
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0952-3278
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16171988-Acetic Acids,
pubmed-meshheading:16171988-Acrylates,
pubmed-meshheading:16171988-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:16171988-Arachidonic Acids,
pubmed-meshheading:16171988-Benzoates,
pubmed-meshheading:16171988-Calcium Signaling,
pubmed-meshheading:16171988-HeLa Cells,
pubmed-meshheading:16171988-Humans,
pubmed-meshheading:16171988-Indoles,
pubmed-meshheading:16171988-Ionomycin,
pubmed-meshheading:16171988-Leukocytes,
pubmed-meshheading:16171988-Leukotriene Antagonists,
pubmed-meshheading:16171988-Leukotriene B4,
pubmed-meshheading:16171988-Phosphonic Acids,
pubmed-meshheading:16171988-Polymerase Chain Reaction,
pubmed-meshheading:16171988-Receptors, Leukotriene B4,
pubmed-meshheading:16171988-Thapsigargin,
pubmed-meshheading:16171988-Thiophenes,
pubmed-meshheading:16171988-Transfection
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pubmed:year |
2005
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pubmed:articleTitle |
Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology.
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pubmed:affiliation |
Division of Physiological Chemistry II, Department of Medical Biochemistry, Biophysics, Karolinska Institute, Sweden. Alan.Sabirsch@ki.se
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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