Source:http://linkedlifedata.com/resource/pubmed/id/16171942
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2005-10-3
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| pubmed:abstractText |
Based on the binding between peroxisome proliferators-activated receptor gamma (PPARgamma) and steroid receptor co-activator-1 (SRC-1), an enzyme-linked immunosorbent assay (ELISA) was used to screen new PPARgamma ligands from various benzylidinethiazole derivatives, which have anti-inflammatory activity. Among those derivatives, 5-(3,5-di-tert-butyl-4-hydroxybenzylidene) thiazolidine-2,4-dione (BTZD) increased the binding between PPARgamma and SRC-1. BTZD was found to induce adipogenesis and PPARgamma trans-activation in 3T3-L1 pre-adipocyte, and increased the binding between PPARgamma and SRC-1 in in vitro binding assay and complex consisting of PPARgamma and SRC-1 in the co-immunoprecipitaion. Chromatin immunoprecipitation (ChIP) analysis revealed that BTZD induced the binding of PPARgamma-SRC-1 complex to PPAR response element (PPRE) in the same pattern of other PPARgamma ligand. From these studies, we have identified and studied the function of a new PPARgamma ligand, BTZD. We suggest that BTZD can be used as a modulator of PPARgamma. This study applying ELISA and ChIP assay can offer new methods to screen PPARgamma ligand and understand the effects of PPARgamma ligands on inflammation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Histone Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Ncoa1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Coactivator 1,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0303-7207
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
20
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| pubmed:volume |
242
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
96-102
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:16171942-3T3-L1 Cells,
pubmed-meshheading:16171942-Adipocytes,
pubmed-meshheading:16171942-Animals,
pubmed-meshheading:16171942-Binding Sites,
pubmed-meshheading:16171942-Fibroblasts,
pubmed-meshheading:16171942-Genes, Reporter,
pubmed-meshheading:16171942-Histone Acetyltransferases,
pubmed-meshheading:16171942-Ligands,
pubmed-meshheading:16171942-Lipid Metabolism,
pubmed-meshheading:16171942-Mice,
pubmed-meshheading:16171942-Nuclear Receptor Coactivator 1,
pubmed-meshheading:16171942-PPAR gamma,
pubmed-meshheading:16171942-Promoter Regions, Genetic,
pubmed-meshheading:16171942-Protein Binding,
pubmed-meshheading:16171942-Recombinant Fusion Proteins,
pubmed-meshheading:16171942-Thiazolidinediones,
pubmed-meshheading:16171942-Transcription Factors,
pubmed-meshheading:16171942-Transcriptional Activation
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| pubmed:year |
2005
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| pubmed:articleTitle |
5-(3,5-Di-tert-butyl-4-hydroxybenzylidene) thiazolidine-2,4-dione modulates peroxisome proliferators-activated receptor gamma in 3T3-L1 adipocytes: roles as a PPARgamma ligand.
|
| pubmed:affiliation |
Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Daejeon 305-600, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|