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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-9-23
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pubmed:abstractText |
Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ANP32A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/AXIN1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Axin Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CRMP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:AbrahamClaudiaC,
pubmed-author:BirchmeierWalterW,
pubmed-author:BockNicoleN,
pubmed-author:BrembeckFelix HFH,
pubmed-author:DroegeAnjaA,
pubmed-author:GoeddeAstridA,
pubmed-author:GoehlerHeikeH,
pubmed-author:HaenigChristianC,
pubmed-author:KietzmannSilviaS,
pubmed-author:KoeppenSusanneS,
pubmed-author:KornBernhardB,
pubmed-author:KrobitschSylviaS,
pubmed-author:LalowskiMaciejM,
pubmed-author:LehrachHansH,
pubmed-author:MintzlaffSaschaS,
pubmed-author:SchoenherrAnkeA,
pubmed-author:StelzlUlrichU,
pubmed-author:StroedickeMartinM,
pubmed-author:TimmJanJ,
pubmed-author:ToksözEnginE,
pubmed-author:WankerErich EEE,
pubmed-author:WormUweU,
pubmed-author:ZenknerMartinaM
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
957-68
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16169070-Axin Protein,
pubmed-meshheading:16169070-Databases as Topic,
pubmed-meshheading:16169070-Humans,
pubmed-meshheading:16169070-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16169070-Models, Molecular,
pubmed-meshheading:16169070-Nerve Tissue Proteins,
pubmed-meshheading:16169070-Protein Binding,
pubmed-meshheading:16169070-Proteins,
pubmed-meshheading:16169070-Proteomics,
pubmed-meshheading:16169070-Repressor Proteins,
pubmed-meshheading:16169070-Two-Hybrid System Techniques
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pubmed:year |
2005
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pubmed:articleTitle |
A human protein-protein interaction network: a resource for annotating the proteome.
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pubmed:affiliation |
Max Delbrueck Center for Molecular Medicine, 13092 Berlin-Buch, Germany.
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pubmed:publicationType |
Journal Article
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