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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1992-8-6
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pubmed:abstractText |
Mildly oxidized low density lipoprotein (minimally modified low density lipoprotein [MM-LDL] is capable of inducing gene expression in cells of the artery wall. In this study, we investigated the mechanisms that control the mRNA expression of JE, KC, c-myc, and c-fos in quiescent mouse L-cell fibroblasts stimulated with MM-LDL. The data demonstrate that MM-LDL induces increases greater than or equal to 20-fold in the levels of transcripts of these genes within 15-60 minutes. Of the four genes examined, JE and KC mRNA showed the greatest response to MM-LDL. The pattern of induction by MM-LDL is distinct from that observed in fibroblasts stimulated with serum, a known inducer of these genes. Treatment with cycloheximide (10 micrograms/ml) did not block the MM-LDL-induced increase in the mRNA levels of these genes. The increase of JE and KC mRNA levels in response to MM-LDL could be blocked by treatment with actinomycin D (5 micrograms/ml). In nuclear runoff studies, MM-LDL increased the transcription rate of JE and KC at 4 hours by 13-fold and fivefold, respectively. Small but reproducible stimulations of c-fos and c-myc transcription by MM-LDL were also observed. In addition, the half-life of JE mRNA was increased after addition of MM-LDL to fibroblasts, suggesting that the MM-LDL-induced accumulation of these mRNAs might be accomplished by both transcriptional and posttranscriptional mechanisms.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1049-8834
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
800-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1616905-Animals,
pubmed-meshheading:1616905-Blood Physiological Phenomena,
pubmed-meshheading:1616905-Cells, Cultured,
pubmed-meshheading:1616905-Fibroblasts,
pubmed-meshheading:1616905-Gene Expression,
pubmed-meshheading:1616905-Gene Expression Regulation,
pubmed-meshheading:1616905-Lipoproteins, LDL,
pubmed-meshheading:1616905-Mice,
pubmed-meshheading:1616905-Protein Kinase C,
pubmed-meshheading:1616905-RNA, Messenger,
pubmed-meshheading:1616905-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1616905-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Mechanisms controlling competence gene expression in murine fibroblasts stimulated with minimally modified LDL.
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pubmed:affiliation |
Department of Biological Chemistry and Medicine, UCLA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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