Source:http://linkedlifedata.com/resource/pubmed/id/16166700
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 5
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pubmed:dateCreated |
2005-9-16
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pubmed:databankReference | |
pubmed:abstractText |
Two moderately halophilic spore-forming bacteria were isolated from salt lakes in the Xinjiang region of China. The two strains, designated AD-6(T) and D-8(T), were aerobic, Gram-positive, rod-shaped and motile by means of peritrichous flagella. Strains AD-6(T) and D-8(T) grew in the presence of 0.5-20% and 0.5-25% (w/v) NaCl in complex medium, respectively. Their cell-wall peptidoglycan was of the L-Orn-D-Asp type. The major menaquinone found in both strains was menaquinone-7 (MK-7). The fatty acid profile contained a large amount of branched fatty acids; the main fatty acids were anteiso-C(15:0), anteiso-C(17:0), iso-C(15:0) and iso-C(16:0). The DNA G+C content of strains D-8(T) and AD-6(T) was 41.4 and 42.2 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains D-8(T) and AD-6(T) were located in the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between the isolated strains and the type strains of Halobacillus species were in the range 96.2-99.5%. DNA-DNA relatedness values of 17.0-52.2% were found between the two strains and other Halobacillus species. The DNA-DNA relatedness value between D-8(T) and AD-6(T) was 50.6%. On the basis of phenotypic and chemotaxonomic properties, phylogenetic analysis and genomic distinctiveness, strains D-8(T) and AD-6(T) should be placed in the genus Halobacillus as two novel species, for which the names Halobacillus dabanensis sp. nov. (type strain=JCM 12772(T)=CGMCC 1.3704(T)) and Halobacillus aidingensis sp. nov. (type strain=JCM 12771(T)=CGMCC 1.3703(T)) are proposed, respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1466-5026
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1991-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16166700-Bacillaceae,
pubmed-meshheading:16166700-Bacterial Typing Techniques,
pubmed-meshheading:16166700-China,
pubmed-meshheading:16166700-DNA, Ribosomal,
pubmed-meshheading:16166700-Fatty Acids,
pubmed-meshheading:16166700-Fresh Water,
pubmed-meshheading:16166700-Molecular Sequence Data,
pubmed-meshheading:16166700-Phenotype,
pubmed-meshheading:16166700-Phylogeny,
pubmed-meshheading:16166700-RNA, Ribosomal, 16S,
pubmed-meshheading:16166700-Sequence Analysis, DNA,
pubmed-meshheading:16166700-Sodium Chloride
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pubmed:year |
2005
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pubmed:articleTitle |
Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China.
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pubmed:affiliation |
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Beijing, 100094, P. R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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