Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2005-9-16
pubmed:abstractText
During formation of spores by Bacillus subtilis the RNA polymerase factor sigma(G) ordinarily becomes active during spore formation exclusively in the prespore upon completion of engulfment of the prespore by the mother cell. Formation and activation of sigma(G) ordinarily requires prior activity of sigma(F) in the prespore and sigma(E) in the mother cell. Here we report that in spoIIA mutants lacking both sigma(F) and the anti-sigma factor SpoIIAB and in which sigma(E) is not active, sigma(G) nevertheless becomes active. Further, its activity is largely confined to the mother cell. Thus, there is a switch in the location of sigma(G) activity from prespore to mother cell. Factors contributing to the mother cell location are inferred to be read-through of spoIIIG, the structural gene for sigma(G), from the upstream spoIIG locus and the absence of SpoIIAB, which can act in the mother cell as an anti-sigma factor to sigma(G). When the spoIIIG locus was moved away from spoIIG to the distal amyE locus, sigma(G) became active earlier in sporulation in spoIIA deletion mutants, and the sporulation septum was not formed, suggesting that premature sigma(G) activation can block septum formation. We report a previously unrecognized control in which SpoIIGA can prevent the appearance of sigma(G) activity, and pro-sigma(E) (but not sigma(E)) can counteract this effect of SpoIIGA. We find that in strains lacking sigma(F) and SpoIIAB and engineered to produce active sigma(E) in the mother cell without the need for SpoIIGA, sigma(G) also becomes active in the mother cell.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-10096082, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-11325926, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-11606741, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-11673427, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12533463, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12591876, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12603732, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12736, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12813085, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-12867473, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-14527287, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-15175314, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-15187183, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-1538747, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-15819616, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-1902213, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-1902463, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-1938874, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-2439490, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-2448286, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-2459711, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-2497051, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-2497052, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-3029030, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-3142866, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-4956288, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-6088672, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-7892217, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-7961403, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-8332059, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-8355618, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-8464402, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-8631668, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166546-8885263
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
187
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6832-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Control of the expression and compartmentalization of (sigma)G activity during sporulation of Bacillus subtilis by regulators of (sigma)F and (sigma)E.
pubmed:affiliation
Department of Microbiology and Immunology, 3400 North Broad Street, Philadelphia, PA 19140, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural