Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-9-16
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC145227, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC145228, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC145261, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC145481, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146763, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146795, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146810, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146811, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146812, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146813, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146950, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC146975, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148082, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148083, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148099, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148100, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148110, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148163, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148234, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148243, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AC148350
pubmed:abstractText
Fluorescent-based high-information-content fingerprinting (HICF) techniques have recently been developed for physical mapping. These techniques make use of automated capillary DNA sequencing instruments to enable both high-resolution and high-throughput fingerprinting. In this article, we report the construction of a whole-genome HICF FPC map for maize (Zea mays subsp. mays cv B73), using a variant of HICF in which a type IIS restriction enzyme is used to generate the fluorescently labeled fragments. The HICF maize map was constructed from the same three maize bacterial artificial chromosome libraries as previously used for the whole-genome agarose FPC map, providing a unique opportunity for direct comparison of the agarose and HICF methods; as a result, it was found that HICF has substantially greater sensitivity in forming contigs. An improved assembly procedure is also described that uses automatic end-merging of contigs to reduce the effects of contamination and repetitive bands. Several new features in FPC v7.2 are presented, including shared-memory multiprocessing, which allows dramatically faster assemblies, and automatic end-merging, which permits more accurate assemblies. It is further shown that sequenced clones may be digested in silico and located accurately on the HICF assembly, despite size deviations that prevent the precise prediction of experimental fingerprints. Finally, repetitive bands are isolated, and their effect on the assembly is studied.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-10087190, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-10391214, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11076862, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11237014, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11386750, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11591643, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11788746, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-11910002, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12481043, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12481051, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12727910, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12853580, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12906862, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-12915486, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-15266340, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-15388850, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-15470886, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-15489523, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-15888684, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-16593771, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-2554336, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-2720459, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-2838135, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-3463999, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-4441361, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-6299666, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-8864112, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-9367125, http://linkedlifedata.com/resource/pubmed/commentcorrection/16166258-9371743
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-38
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Whole-genome validation of high-information-content fingerprinting.
pubmed:affiliation
Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, 85721, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.