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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2005-9-14
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pubmed:abstractText |
Retroviral integrases (INs) function in the context of preintegration complexes (PICs). Two conserved Lys residues in the N-terminal domain of human immunodeficiency virus type 1 (HIV-1) IN were analyzed here for their roles in integration and virus replication. Whereas HIV-1(K46A) grew like the wild type, HIV-1(K34A) was dead. Yet recombinant IN(K34A) protein functioned in in vitro integration assays, and Vpr-IN(K34A) efficiently transcomplemented the infectivity defect of an IN active site mutant virus in cells. HIV-1(K34A) was therefore similar to a number of previously characterized mutant viruses that failed to replicate despite encoding catalytically competent IN. To directly analyze mutant PIC function, a sensitive PCR-based integration assay was developed. HIV-1(K34A) and related mutants failed to support detectable levels (<1% of wild type) of integration. We therefore concluded that mutations like K34A disrupted higher-order interactions important for PIC function/maturation compared to the innate catalytic activity of IN enzyme.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10358097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10384245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10385323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10516007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10649997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10669606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10669607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-10890912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11389849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11406603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11483739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11564861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11595745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-11743009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-12368302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-12414950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-12670953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-1404595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-15353349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-15542626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-15681450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-1577801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-16051828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-1847518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-2167180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-2170022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-2335814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-2349226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-2539592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-3032450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-3401925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-7535863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-7688824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-7852375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8344263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8344264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8371356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8386373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8420982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8551608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8676485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8942990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-8993331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-9228950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-9305652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-9305653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16160186-9368756
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12584-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2005
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pubmed:articleTitle |
Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication.
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pubmed:affiliation |
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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