Linked Life Data

1615735

Source:http://linkedlifedata.com/resource/pubmed/id/1615735

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-7-28
pubmed:abstractText
Antinuclear antibodies are of major importance in the diagnosis of inflammatory rheumatic diseases. Sm and nRNP antibodies can be found in sera from patients with systemic lupus erythematosus and mixed connective tissue disease. Usually, these antibodies have been detected with one of the following methods: Ouchterlony immunodiffusion, passive hemagglutination or counterimmunoelectrophoresis (CIE). In this work results obtained by Ouchterlony and CIE techniques were compared with those obtained by ELISA. Purified proteins from cellular extracts (HeLa) were used as antigens for Sm- and nRNP-ELISA: D polypeptide for Sm-ELISA and the 68 kD, A, C, B,B' and D polypeptides for nRNP-ELISA. Compared with the other two techniques, ELISA was less time consuming and showed greater sensitivity. Quantitative titration proved to be of advantage in monitoring the course of the diseases mentioned above.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0340-1855
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-93
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
[Enzyme immunoassay for the detection of autoantibodies to nRNP and Sm: a rapid and sensitive alternative to current procedures].
pubmed:affiliation
Zentrallabor der Medizinischen Klinik und Poliklinik Heidelberg.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract, Research Support, Non-U.S. Gov't