| pubmed-article:16156856 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16156856 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:16156856 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:16156856 | lifeskim:mentions | umls-concept:C0003993 | lld:lifeskim |
| pubmed-article:16156856 | lifeskim:mentions | umls-concept:C0205296 | lld:lifeskim |
| pubmed-article:16156856 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16156856 | pubmed:dateCreated | 2005-9-13 | lld:pubmed |
| pubmed-article:16156856 | pubmed:abstractText | We examined the effectiveness of various anti-tumour agents to natural killer (NK)-cell tumour cell lines and samples, which are generally resistant to chemotherapy, using flow cytometric terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling (TUNEL) assay. Although NK-YS and NK-92 were highly resistant to various anti-tumour agents, l-asparaginase induced apoptosis in these two NK-cell lines. NK-cell leukaemia/lymphoma and acute lymphoblastic leukaemia (ALL) samples were selectively sensitive to l-asparaginase and to doxorubicin (DXR) respectively. Samples of chronic NK lymphocytosis, an NK-cell disorder with an indolent clinical course, were resistant to both drugs. Our study clearly separated two major categories of NK-cell disorders and ALL according to the sensitivity to DXR and l-asparaginase. We examined asparagine synthetase levels by real-time quantitative polymerase chain reaction (RQ-PCR) and immunostaining in these samples. At least in nasal-type NK-cell lymphoma, there was a good correlation among asparagine synthetase expression, in vitro sensitivity and clinical response to l-asparaginase. In aggressive NK-cell leukaemia, although asparagine synthetase expression was high at both mRNA and protein levels, l-asparaginase induced considerable apoptosis. Furthermore, samples of each disease entity occupied a distinct area in two-dimensional plotting with asparagine synthetase mRNA level (RQ-PCR) and in vitrol-asparaginase sensitivity (TUNEL assay). We confirmed rather specific anti-tumour activity of l-asparaginase against NK-cell tumours in vitro, which provides an experimental background to the clinical use of l-asparaginase for NK-cell tumours. | lld:pubmed |
| pubmed-article:16156856 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16156856 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16156856 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16156856 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16156856 | pubmed:issn | 0007-1048 | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:SasakiMakotoM | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:SugimotoKoich... | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:OshimiKazuoK | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:KitohToshiyuk... | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:EgashiraMotok... | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:SchusterSheld... | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:AndoMikiM | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:AndoJunJ | lld:pubmed |
| pubmed-article:16156856 | pubmed:author | pubmed-author:MukaiKouichiK | lld:pubmed |
| pubmed-article:16156856 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16156856 | pubmed:volume | 130 | lld:pubmed |
| pubmed-article:16156856 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16156856 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16156856 | pubmed:pagination | 860-8 | lld:pubmed |
| pubmed-article:16156856 | pubmed:meshHeading | pubmed-meshheading:16156856... | lld:pubmed |
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| pubmed-article:16156856 | pubmed:meshHeading | pubmed-meshheading:16156856... | lld:pubmed |
| pubmed-article:16156856 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16156856 | pubmed:articleTitle | Selective apoptosis of natural killer-cell tumours by l-asparaginase. | lld:pubmed |
| pubmed-article:16156856 | pubmed:affiliation | Department of Haematology, Juntendo University School of Medicine, Tokyo, Japan. | lld:pubmed |
| pubmed-article:16156856 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16156856 | lld:pubmed |
