Source:http://linkedlifedata.com/resource/pubmed/id/16156856
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2005-9-13
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pubmed:abstractText |
We examined the effectiveness of various anti-tumour agents to natural killer (NK)-cell tumour cell lines and samples, which are generally resistant to chemotherapy, using flow cytometric terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling (TUNEL) assay. Although NK-YS and NK-92 were highly resistant to various anti-tumour agents, l-asparaginase induced apoptosis in these two NK-cell lines. NK-cell leukaemia/lymphoma and acute lymphoblastic leukaemia (ALL) samples were selectively sensitive to l-asparaginase and to doxorubicin (DXR) respectively. Samples of chronic NK lymphocytosis, an NK-cell disorder with an indolent clinical course, were resistant to both drugs. Our study clearly separated two major categories of NK-cell disorders and ALL according to the sensitivity to DXR and l-asparaginase. We examined asparagine synthetase levels by real-time quantitative polymerase chain reaction (RQ-PCR) and immunostaining in these samples. At least in nasal-type NK-cell lymphoma, there was a good correlation among asparagine synthetase expression, in vitro sensitivity and clinical response to l-asparaginase. In aggressive NK-cell leukaemia, although asparagine synthetase expression was high at both mRNA and protein levels, l-asparaginase induced considerable apoptosis. Furthermore, samples of each disease entity occupied a distinct area in two-dimensional plotting with asparagine synthetase mRNA level (RQ-PCR) and in vitrol-asparaginase sensitivity (TUNEL assay). We confirmed rather specific anti-tumour activity of l-asparaginase against NK-cell tumours in vitro, which provides an experimental background to the clinical use of l-asparaginase for NK-cell tumours.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Asparaginase,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartate-Ammonia Ligase,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1048
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
860-8
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pubmed:meshHeading |
pubmed-meshheading:16156856-Antineoplastic Agents,
pubmed-meshheading:16156856-Apoptosis,
pubmed-meshheading:16156856-Asparaginase,
pubmed-meshheading:16156856-Aspartate-Ammonia Ligase,
pubmed-meshheading:16156856-Cell Line, Tumor,
pubmed-meshheading:16156856-Doxorubicin,
pubmed-meshheading:16156856-Drug Resistance, Neoplasm,
pubmed-meshheading:16156856-Humans,
pubmed-meshheading:16156856-In Situ Nick-End Labeling,
pubmed-meshheading:16156856-Killer Cells, Natural,
pubmed-meshheading:16156856-Leukemia, T-Cell,
pubmed-meshheading:16156856-Lymphocytosis,
pubmed-meshheading:16156856-Lymphoma, T-Cell,
pubmed-meshheading:16156856-RNA, Messenger,
pubmed-meshheading:16156856-RNA, Neoplasm
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pubmed:year |
2005
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pubmed:articleTitle |
Selective apoptosis of natural killer-cell tumours by l-asparaginase.
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pubmed:affiliation |
Department of Haematology, Juntendo University School of Medicine, Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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