Source:http://linkedlifedata.com/resource/pubmed/id/16153078
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2005-9-12
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| pubmed:abstractText |
A novel cytoplasmic delivery system of antisense oligodeoxynucleotide (asODN) was developed by assembling a PEG-asODN conjugate with disulfide linkage (smart linkage) (PEG-SS-asODN) into polyion complex (PIC) micelles through the complexation with branched poly(ethylenimine) (B-PEI). The PIC micelle thus prepared showed a significant antisense effect against luciferase gene expression in HuH-7 cells, far more efficient than nonmicelle systems (asODN and PEG-SS-asODN in free form) and PIC micelle encapsulating the conjugate without the disulfide linkage. Use of poly(l-lysine) (PLL) instead of the B-PEI for PIC micellization led to a substantial decrease in the antisense effect. These results indicate that the PIC micelles formulated from PEG-SS-asODN conjugate and B-PEI is successfully transported from the endosomal compartment into the cytoplasm by the buffering effect of the B-PEI, releasing hundreds of active asODN molecules via cleavage of the disulfide linkage into the cellular interior, responding to a high glutathione concentration in the cytoplasmic compartment. Furthermore, the type of smart linkage (glutathione-sensitive SS linkage vs pH-sensitive linkage) in the conjugates substantially affected the antisense effect of the PIC micelles, depending on the nature of the counter polycation (B-PEI vs PLL).
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Cations,
http://linkedlifedata.com/resource/pubmed/chemical/Disulfides,
http://linkedlifedata.com/resource/pubmed/chemical/Ions,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Micelles,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Polylysine
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1525-7797
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2449-54
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16153078-Biocompatible Materials,
pubmed-meshheading:16153078-Carcinoma, Hepatocellular,
pubmed-meshheading:16153078-Cations,
pubmed-meshheading:16153078-Cell Line, Tumor,
pubmed-meshheading:16153078-Chromatography,
pubmed-meshheading:16153078-Cytoplasm,
pubmed-meshheading:16153078-Disulfides,
pubmed-meshheading:16153078-Genes, Reporter,
pubmed-meshheading:16153078-Humans,
pubmed-meshheading:16153078-Hydrogen-Ion Concentration,
pubmed-meshheading:16153078-Ions,
pubmed-meshheading:16153078-Luciferases,
pubmed-meshheading:16153078-Micelles,
pubmed-meshheading:16153078-Models, Biological,
pubmed-meshheading:16153078-Models, Chemical,
pubmed-meshheading:16153078-Oligonucleotides, Antisense,
pubmed-meshheading:16153078-Polyethylene Glycols,
pubmed-meshheading:16153078-Polylysine
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| pubmed:articleTitle |
Supramolecular assemblies for the cytoplasmic delivery of antisense oligodeoxynucleotide: polyion complex (PIC) micelles based on poly(ethylene glycol)-SS-oligodeoxynucleotide conjugate.
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| pubmed:affiliation |
Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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