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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-9-8
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pubmed:abstractText |
We recently reported that soluble 60-kDa heat shock protein (HSP60) can directly activate T cells via TLR2 signaling to enhance their Th2 response. In this study we investigated whether HSP60 might also activate B cells by an innate signaling pathway. We found that human HSP60 (but not the Escherichia coli GroEL or the Mycobacterial HSP65 molecules) induced naive mouse B cells to proliferate and to secrete IL-10 and IL-6. In addition, the HSP60-treated B cells up-regulated their expression of MHC class II and accessory molecules CD69, CD40, and B7-2. We tested the functional ability of HSP60-treated B cells to activate an allogeneic T cell response and found enhanced secretion of both IL-10 and IFN-gamma by the responding T cells. The effects of HSP60 were found to be largely dependent on TLR4 and MyD88 signaling; B cells from TLR4-mutant mice or from MyD88 knockout mice showed decreased responses to HSP60. Care was taken to rule out contamination of the HSP60 with LPS as a causative factor. These findings add B cells to the complex web of interactions by which HSP60 can regulate immune responses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonin 60,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/MYD88 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Myd88 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myeloid Differentiation Factor 88,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3594-602
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16148103-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:16148103-Animals,
pubmed-meshheading:16148103-Antigens, Differentiation,
pubmed-meshheading:16148103-B-Lymphocytes,
pubmed-meshheading:16148103-Cell Proliferation,
pubmed-meshheading:16148103-Chaperonin 60,
pubmed-meshheading:16148103-Histocompatibility Antigens Class II,
pubmed-meshheading:16148103-Humans,
pubmed-meshheading:16148103-Interleukins,
pubmed-meshheading:16148103-Lymphocyte Activation,
pubmed-meshheading:16148103-Mice,
pubmed-meshheading:16148103-Mice, Inbred Strains,
pubmed-meshheading:16148103-Mice, Knockout,
pubmed-meshheading:16148103-Myeloid Differentiation Factor 88,
pubmed-meshheading:16148103-Receptors, Immunologic,
pubmed-meshheading:16148103-Signal Transduction,
pubmed-meshheading:16148103-Toll-Like Receptor 4,
pubmed-meshheading:16148103-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.
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pubmed:affiliation |
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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