Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2005-12-5
pubmed:abstractText
Means to prevent thrombus extension and local recurrence remain suboptimal, in part because of the limited effectiveness of existing thrombolytics. In theory, plasminogen activators could be used for this purpose if they could be anchored to the vascular lumen by targeting stably expressed, noninternalized determinants such as platelet-endothelial-cell adhesion molecule 1 (PECAM-1). We designed a recombinant molecule fusing low-molecular-weight single-chain prourokinase plasminogen activator (lmw-scuPA) with a single-chain variable fragment (scFv) of a PECAM-1 antibody to generate the prodrug scFv/lmw-scuPA. Cleavage by plasmin generated fibrinolytically active 2-chain lmw-uPA. This fusion protein (1) bound specifically to PECAM-1-expressing cells; (2) was rapidly cleared from blood after intravenous injection; (3) accumulated in the lungs of wild-type C57BL6/J, but not PECAM-1 null mice; and (4) lysed pulmonary emboli formed subsequently more effectively than lmw-scuPA, thereby providing support for the concept of thromboprophylaxis using recombinant scFv-fibrinolytic fusion proteins that target endothelium.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-10051650, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-10072554, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-10368124, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-10715263, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11156957, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11196310, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11329062, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11350815, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11839549, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-11861781, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12149463, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12244306, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12461559, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12531816, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12640043, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12845330, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-12881310, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-14500967, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-14610017, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15099287, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15144574, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15190345, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15320826, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15543321, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15598644, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-15770007, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-1918077, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-1946453, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-2953770, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-3097021, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-3116546, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-3790724, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-4023710, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-7542999, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-7559536, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-7671373, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8100296, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8174104, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8248808, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8286392, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8930213, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-8999802, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-9024162, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-9415290, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-9461198, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-9664085, http://linkedlifedata.com/resource/pubmed/commentcorrection/16144802-9927694
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4191-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...