Linked Life Data

16143415

Source:http://linkedlifedata.com/resource/pubmed/id/16143415

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-10-21
pubmed:abstractText
Previous studies have revealed distribution of histaminergic fibers and presence of histamine receptors in globus pallidus (GP). In this study, the brain slice preparation of adult rats was used to examine the effect of histamine on the spontaneous unitary discharge of GP neurons and the underlying receptor mechanism. Ninety-five GP neurons were extracellularly recorded from 42 slices containing the GP, of which 87 (91.6%) were excited by the stimulation of histamine. The histamine-induced excitation was concentration-dependent and persisted in low Ca2+/high Mg2+ medium (n = 9), demonstrating that the action of histamine on the GP neurons was postsynaptic. The excitatory effect of histamine on the GP neurons was not blocked by selective histamine H1 receptor antagonist triprolidine (n = 16) or chlorpheniramine (n = 6), but was effectively suppressed by ranitidine, a highly selective histamine H2 receptor antagonist (n = 21). On the other hand, highly selective histamine H2 receptor agonist dimaprit mimicked the excitatory effect of histamine on the GP neurons (n = 23), while histamine H1 receptor agonists, including 2-pyridylethylamine (n = 22), 2-thiazolyethylamine (n = 9) and betahistine (n = 9), did not cause GP neurons any response. The dimaprit-induced GP neuronal excitation was effectively antagonized by selective histamine H2 receptor antagonist ranitidine (n = 14) but not influenced by selective histamine H1 receptor antagonist triprolidine (n = 12). Moreover, adenylate cyclase (AC) activator forskolin (n = 7) was observed to evoke GP neurons an excitatory response, whereas the histamine-induced excitation was effectively reduced by H-89 (n = 9), a selective and potent inhibitor of protein kinase A (PK(A)). Finally, it was noted that neurons of both subdivisions of the GP, the internal (GPi, n = 35) and external (GPe, n = 60) segment, showed no differences in their responses to stimulations of the tested histaminergic reagents. These results demonstrated that histamine excited GP (including GPi and GPe) neurons via histamine H2 receptors and H2 receptors linked intracellular G-protein-AC-PK(A) signaling pathway, suggesting that the hypothalamic histaminergic afferent fibers innervating GP may play an important modulatory role in motor control through its excitatory effect on GP neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0168-0102
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-97
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16143415-Action Potentials, pubmed-meshheading:16143415-Adenylate Cyclase, pubmed-meshheading:16143415-Animals, pubmed-meshheading:16143415-Basal Ganglia Diseases, pubmed-meshheading:16143415-Calcium, pubmed-meshheading:16143415-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:16143415-Dose-Response Relationship, Drug, pubmed-meshheading:16143415-Female, pubmed-meshheading:16143415-Globus Pallidus, pubmed-meshheading:16143415-Histamine, pubmed-meshheading:16143415-Histamine Agonists, pubmed-meshheading:16143415-Histamine Antagonists, pubmed-meshheading:16143415-Magnesium, pubmed-meshheading:16143415-Male, pubmed-meshheading:16143415-Neural Pathways, pubmed-meshheading:16143415-Neurons, pubmed-meshheading:16143415-Organ Culture Techniques, pubmed-meshheading:16143415-Rats, pubmed-meshheading:16143415-Rats, Sprague-Dawley, pubmed-meshheading:16143415-Receptors, Histamine H2, pubmed-meshheading:16143415-Synaptic Transmission
pubmed:year
2005
pubmed:articleTitle
Excitatory effect of histamine on neuronal activity of rat globus pallidus by activation of H2 receptors in vitro.
pubmed:affiliation
Department of Biological Science and Technology and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Mailbox 426, Nanjing University, Nanjing 210093, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't