Linked Life Data

16143391

Source:http://linkedlifedata.com/resource/pubmed/id/16143391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-10-25
pubmed:abstractText
In this study, the effect of cell alignment on proliferation and phenotype expression of rat bone marrow derived osteoblasts on micropatterned (MP) PHBV and P(L/D,L)LA films with 27 microm wide parallel microgrooves was investigated. Immobilization of fibrinogen (Fb) on film surface by adsorption increased hydrophilicity, while covalent immobilization decreased it. Amount of Fb immobilized was significantly higher upon covalent bonding (153.1+/-42.4 microg Fb/cm2) than when adsorbed (10.0+/-3.3 microg Fb/cm2). It was observed that the presence of MP did not influence cell proliferation in the long run. Osteoblasts on MP films with adsorbed (MP Fb(a)) and covalently immobilized Fb (MP Fb(i)) aligned parallel to the groove axis with mean deviation angles of 10.59+/-23.47 and 29.02+/-33.03 degrees, respectively, while on tissue culture polystyrene (TCP), on unpatterned films (UNP) and on UNP with adsorbed Fb (UNP Fb(a)) alignment with an arbitrary axis was much higher: 46.66+/-24.98, 48.72+/-31.19, 47.74+/-27.29 degrees, respectively. Fb-free MP films were not effective in cell alignment, and clumps were formed. Cell alignment achieved on MP Fb(a) films did not influence cell proliferation, but increased differentiation, as shown by ALP activity per cell and the evenness and the amount of calcium phosphate deposition. It was concluded that orientation of cells was influential on their differentiation and also, MP cell carriers with chemical cues on their surfaces are important in improving tissue repair.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
885-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Tissue engineering of bone on micropatterned biodegradable polyester films.
pubmed:affiliation
Biotechnology Research Unit, Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey. hkenar@metu.edu.tr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't