Source:http://linkedlifedata.com/resource/pubmed/id/16143313
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2005-10-3
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| pubmed:abstractText |
In this study, we demonstrate that N-ethylmaleimide (NEM), a cell permeable thiol-alkylating agent, enhanced the [Ca2+]i rise caused by stimulation with cyclopiazonic acid (CPA), a sarcoplasmic-endoplasmic reticulum Ca2+-ATPase inhibitor, in rat neutrophils. In addition, NEM attenuated the formyl-Met-Leu-Phe (fMLP)-induced [Ca2+]i rise whether NEM was added to cells prior to or after fMLP stimulation. Moreover, application of NEM after fMLP activation in the absence of external Ca2+ inhibited the Ca2+ signal upon addition of Ca2+ to the medium. Similar patterns were also obtained by using 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), a cell impermeable dithiol-oxidizing agent, which replaced NEM in the CPA- and fMLP-induced [Ca2+]i rise experiments. Treatment with dithiothreitol (DTT), a cell permeable dithiol-reducing agent, N-acetyl-l-cysteine (NAC), a cell permeable monothiol-reducing agent, and tris-(2-carboxyethyl)phosphine (TCEP), a cell impermeable reductant without a thiol group, all rescued the fMLP-induced Ca2+ signal from NEM. Rat neutrophils express the mRNA encoding for transient receptor potential (TRP) C6, inositol trisphosphate receptor (IP3R) 2 and IP3R3. NEM had no effect on the mitochondrial membrane potential. NEM could restore the polarization and F-actin accumulation of fMLP-treated cells to those of the control. In the absence of external Ca2+, NEM rendered the CPA-induced [Ca2+]i elevation persistently but inhibited the fMLP-induced Ca2+ spike, which was reversed by tris-(2-cyanoethyl)phosphine (TCP), a cell permeable reductant without a thiol group. DTNB did not affect the Ca2+ spike caused by fMLP. These results indicate that through protein thiol oxidation, NEM affects the receptor-activated and the store depletion-derived Ca2+ signals in an opposing manner.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Dithionitrobenzoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/TRPC Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Trrp6 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/cyclopiazonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/tris(2-carboxyethyl)phosphine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0006-2952
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
70
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1320-9
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| pubmed:dateRevised |
2007-7-18
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| pubmed:meshHeading |
pubmed-meshheading:16143313-Actins,
pubmed-meshheading:16143313-Animals,
pubmed-meshheading:16143313-Calcium Channels,
pubmed-meshheading:16143313-Calcium Signaling,
pubmed-meshheading:16143313-Dithionitrobenzoic Acid,
pubmed-meshheading:16143313-Ethylmaleimide,
pubmed-meshheading:16143313-Indoles,
pubmed-meshheading:16143313-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:16143313-Male,
pubmed-meshheading:16143313-Membrane Potentials,
pubmed-meshheading:16143313-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:16143313-Neutrophils,
pubmed-meshheading:16143313-Phosphines,
pubmed-meshheading:16143313-RNA, Messenger,
pubmed-meshheading:16143313-Rats,
pubmed-meshheading:16143313-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:16143313-Sulfhydryl Compounds,
pubmed-meshheading:16143313-TRPC Cation Channels
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| pubmed:year |
2005
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| pubmed:articleTitle |
Distinct effects of N-ethylmaleimide on formyl peptide- and cyclopiazonic acid-induced Ca2+ signals through thiol modification in neutrophils.
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| pubmed:affiliation |
Department of Biochemistry, School of Medicine, China Medical University, Taichung, Taiwan, ROC.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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