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rdf:type |
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lifeskim:mentions |
umls-concept:C0035696,
umls-concept:C0036003,
umls-concept:C0043342,
umls-concept:C0162638,
umls-concept:C0205087,
umls-concept:C0205263,
umls-concept:C0220781,
umls-concept:C0332152,
umls-concept:C0667830,
umls-concept:C1136028,
umls-concept:C1412379,
umls-concept:C1705848,
umls-concept:C1879547,
umls-concept:C1883254,
umls-concept:C2745955
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pubmed:issue |
2
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pubmed:dateCreated |
2005-9-16
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pubmed:abstractText |
Overexpression of S-adenosylmethionine decarboxylase (SAMDC) in Xenopus fertilized eggs activates caspase-9 and executes maternal program of apoptosis shortly after midblastula transition (MBT). We find that overexpression of caspase-8 and p53, like that of SAMDC, induces apoptosis in Xenopus late blastulae. The apoptosis induced by p53 was abolished by injection of mRNA for xdm-2, a negative regulator of p53, and by injection of a peptide inhibitor or a dominant-negative type mutant of caspase-9, but not caspase-8. The apoptosis induced by SAMDC was not abolished by injection of xdm-2 mRNA, but was abolished by injection of a peptide inhibitor or a dominant-negative type mutant mRNA of both caspase-9 and caspase-8. Unlike caspase-9 mRNA, caspase-8 mRNA did not occur as a maternal mRNA rather induced to be expressed during cleavage stage (pre-MBT stage) by overexpression of SAMDC but not p53. Furthermore, while activities to process procaspase-8 and procaspase-9 appeared in SAMDC-overexpressed apoptotic embryos, the activity to process procaspase-8 did not appear in p53-overexpressed apoptotic embryos. We conclude there are at least two pathways in the execution of the maternal program of apoptosis in Xenopus embryos; one being through do novo expression of caspase-8 gene during cleavage stage, and the other without involvement of caspase-8.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:HaraHiroshiH,
pubmed-author:HigoTakayasuT,
pubmed-author:IgarashiKazueiK,
pubmed-author:KaitoChikaraC,
pubmed-author:KajitaniMasayukiM,
pubmed-author:KuroyanagiShinsakuS,
pubmed-author:MiuraKin-IchiroK,
pubmed-author:SekimizuKazuhisaK,
pubmed-author:ShiokawaKoichiroK,
pubmed-author:TadakumaTakushiT,
pubmed-author:TakayamaEijiE,
pubmed-author:YaoitaYoshioY
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
336
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
682-91
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16143307-Adenosylmethionine Decarboxylase,
pubmed-meshheading:16143307-Animals,
pubmed-meshheading:16143307-Apoptosis,
pubmed-meshheading:16143307-Blastula,
pubmed-meshheading:16143307-Caspase 8,
pubmed-meshheading:16143307-Caspases,
pubmed-meshheading:16143307-Enzyme Activation,
pubmed-meshheading:16143307-Gene Expression Regulation, Developmental,
pubmed-meshheading:16143307-Plant Proteins,
pubmed-meshheading:16143307-RNA, Messenger,
pubmed-meshheading:16143307-Recombinant Proteins,
pubmed-meshheading:16143307-Tumor Suppressor Protein p53,
pubmed-meshheading:16143307-Xenopus laevis
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pubmed:year |
2005
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pubmed:articleTitle |
Occurrence of pre-MBT synthesis of caspase-8 mRNA and activation of caspase-8 prior to execution of SAMDC (S-adenosylmethionine decarboxylase)-induced, but not p53-induced, apoptosis in Xenopus late blastulae.
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pubmed:affiliation |
Laboratory of Molecular Embryology, Department of Biological Science, Graduate School of Sciences, The University of Tokyo, 7-3 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. shiokawa@nasu.bio.teikyo-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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