Source:http://linkedlifedata.com/resource/pubmed/id/16138266
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2005-9-2
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| pubmed:abstractText |
Glucose-free perfusion preconditions myocardium against the consequences of subsequent ischemia. We investigated whether mitochondrial ATP-sensitive potassium (mK (ATP)) channels are involved in preconditioning by glucose deprivation, and whether moderate glucose deprivation also preconditions myocardium. Isolated rat hearts underwent 30 min of no-flow ischemia followed by 1 h reperfusion. Controls were not further treated. Three groups were preconditioned by perfusion with 0, 40 or 80 mg/dl (0, 2.22, 4.44 mmol/l) glucose (correction of osmotic pressure by addition of urea) for 10 min followed by 10 min perfusion with normal buffer (150 mg/dl, or 8.33 mmol/l glucose) before the ischemia reperfusion protocol. In one group, 100 micromol/l of the mK (ATP) channel blocker 5-HD was added to the glucose-free perfusate. Two groups were treated with 5-HD or urea before ischemia without preconditioning. Left ventricular developed pressure and maximum ischemic contracture (82 +/- 21 mmHg) were similar in all groups. Mean left ventricular developed pressure was 100 +/- 16 mm Hg under baseline conditions, and poorly recovered to 8 +/- 11 mm Hg during reperfusion. Preconditioning with 0 and 40 mg/dl glucose containing buffer reduced infarct size from 41 +/- 10% (control) to 23 +/- 12% (p = 0.02) and 26 +/- 8% (p = 0.011). The 5-HD blocked preconditioning by glucose deprivation (38 +/- 9%, p = 0.04) while 80 mg/dl glucose, 5-HD and urea had no effect on infarct size (39 +/- 9%; 38 +/- 13%; 37 +/- 8%; p = 1.0 each). We conclude that transient severe glucose deprivation and moderate glucose deprivation preconditions the isolated rat heart. Preconditioning by complete glucose deprivation depends on the opening of mK (ATP) channels.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0018-5043
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
37
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
516-20
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| pubmed:dateRevised |
2009-2-19
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| pubmed:meshHeading |
pubmed-meshheading:16138266-Animals,
pubmed-meshheading:16138266-Glucose,
pubmed-meshheading:16138266-Myocardial Infarction,
pubmed-meshheading:16138266-Myocardial Reperfusion,
pubmed-meshheading:16138266-Myocardial Reperfusion Injury,
pubmed-meshheading:16138266-Myocardium,
pubmed-meshheading:16138266-Organ Culture Techniques,
pubmed-meshheading:16138266-Potassium Channel Blockers,
pubmed-meshheading:16138266-Potassium Channels,
pubmed-meshheading:16138266-Rats,
pubmed-meshheading:16138266-Urea
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| pubmed:year |
2005
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| pubmed:articleTitle |
Moderate glucose deprivation preconditions myocardium against infarction.
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| pubmed:affiliation |
Klinik für Anästhesiologie, Universitätsklinikum Düsseldorf, Düsseldorf, Germany. ebeld@uni-duesseldorf.de
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| pubmed:publicationType |
Journal Article
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