Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-10-11
pubmed:abstractText
Feline brain endothelial cells (BECs), astrocytes, and microglia were combined in different configurations in a cell culture insert system to assess the effect of different cell types on the trafficking of peripheral blood mononuclear cell (PBMC) subsets in response to feline immunodeficiency virus (FIV). The addition of astrocytes to BECs significantly increased the adherence of PBMCs. This increase in adherence was suppressed by microglia, whereas microglia alone had no effect on PBMC adherence. FIV exposure of the glial cells did not alter PBMC adherence as compared to same configurations with untreated cells. All PBMC subsets showed some level of trafficking across the endothelial cell layer. The level of trafficking of monocytes and B cells was significantly increased if astrocytes were present. The presence of microglia with the astrocytes reduced transmigration across all PBMC subsets. FIV exposure of astrocytes significantly increased the percentage of CD8 T cell transmigration from 24% to 64% of the total CD4 and CD8 numbers. The presence of microglia significantly reversed the preferential trafficking of CD8 cells in the presence of astrocytes. The results suggested that interaction between the triad of endothelial cells, astrocytes, and microglia played an important, but varying, role in the trafficking of different PBMC subsets. In general, astrocytes had a positive effect on trafficking of PBMCs, while microglia had a suppressive effect. Effects of FIV on trafficking were largely restricted to increases seen in CD8 T cells and monocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1872-6240
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
1058
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148-60
pubmed:meshHeading
pubmed-meshheading:16137663-Animals, pubmed-meshheading:16137663-Astrocytes, pubmed-meshheading:16137663-B-Lymphocytes, pubmed-meshheading:16137663-Blood-Brain Barrier, pubmed-meshheading:16137663-Brain, pubmed-meshheading:16137663-Cats, pubmed-meshheading:16137663-Cell Communication, pubmed-meshheading:16137663-Cells, Cultured, pubmed-meshheading:16137663-Cerebral Arteries, pubmed-meshheading:16137663-Chemotaxis, Leukocyte, pubmed-meshheading:16137663-Coculture Techniques, pubmed-meshheading:16137663-Endothelial Cells, pubmed-meshheading:16137663-Feline Acquired Immunodeficiency Syndrome, pubmed-meshheading:16137663-Immune Tolerance, pubmed-meshheading:16137663-Immunodeficiency Virus, Feline, pubmed-meshheading:16137663-Lymphocytes, pubmed-meshheading:16137663-Microglia, pubmed-meshheading:16137663-Monocytes, pubmed-meshheading:16137663-T-Lymphocytes
pubmed:year
2005
pubmed:articleTitle
Astrocytes and microglia differentially regulate trafficking of lymphocyte subsets across brain endothelial cells.
pubmed:affiliation
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA. lola_hudson@ncsu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural