Linked Life Data

16126358

Source:http://linkedlifedata.com/resource/pubmed/id/16126358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-1-13
pubmed:abstractText
A highly selective and sensitive HPLC-ESI-MS-MS method was developed for the determination of oleanolic acid in human plasma. The oleanolic acid and glycyrrhetinic acid (internal standard) were recovered from plasma with ethyl acetate liquid-liquid extraction. The organic extracts were dried under a stream of warm nitrogen, reconstituted in mobile phase and injected into a Zorbax-Extend ODS analytical column (150 mm x 4.6 mm i.d., 5 microm), with the mobile phase consisting of methanol-ammonium acetate (32.5 mM) (85:15, v/v) pumped at a flow rate of 1.0 ml/min, and 30% of the eluent was split into a MS system with electrospray ionization tandem mass (ESI-MS-MS) detection in negative ion mode. The tandem mass detection was performed on a Finnigan Surveyor LC-TSQ Quantum Ultra AM tandem mass spectrometer operated in selected reaction monitoring mode. The parent to product ion combinations of m/z 455.4-->455.4 and 469.3-->425.2 at 38 V 1.5 mTorr Ar CID were used to quantify oleanolic acid and glycyrrhetinic acid, respectively. The assay was validated in the concentration range of 0.02-30.0 ng/ml for oleacolic acid when 0.5 ml of plasma was processed. The precision of the assay (expressed as relative standard deviation, R.S.D.%) was less than 15% at all concentrations levels within the tested range and adequate accuracy, and the limit of quantification was 0.02 ng/ml. The established method was applied for the pharmacokinetics study of oleanolic acid capsules in 18 healthy male Chinese volunteers with the mean values of C(max), T(max), AUC(0-48), AUC(0-infinity), t(1/2,) CL/F, and V/F of oleanolic acid after p.o. a single 40 mg dose obtained were 12.12 +/- 6.84 ng/ml, 5.2 +/- 2.9h, 114.34 +/- 74.87 ng h/ml, 124.29 +/- 106.77 ng h/ml, 8.73 +/- 6.11 h, 555.3 +/- 347.7 L/h, and 3371.1 +/- 1,990.1 L, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
190-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16126358-Acetates, pubmed-meshheading:16126358-Adult, pubmed-meshheading:16126358-Area Under Curve, pubmed-meshheading:16126358-Calibration, pubmed-meshheading:16126358-Chemistry, Pharmaceutical, pubmed-meshheading:16126358-Chemistry Techniques, Analytical, pubmed-meshheading:16126358-China, pubmed-meshheading:16126358-Chromatography, High Pressure Liquid, pubmed-meshheading:16126358-Dose-Response Relationship, Drug, pubmed-meshheading:16126358-Glycyrrhetinic Acid, pubmed-meshheading:16126358-Humans, pubmed-meshheading:16126358-Male, pubmed-meshheading:16126358-Methanol, pubmed-meshheading:16126358-Models, Chemical, pubmed-meshheading:16126358-Oleanolic Acid, pubmed-meshheading:16126358-Reproducibility of Results, pubmed-meshheading:16126358-Spectrometry, Mass, Electrospray Ionization, pubmed-meshheading:16126358-Time Factors
pubmed:year
2006
pubmed:articleTitle
Determination of oleanolic acid in human plasma and study of its pharmacokinetics in Chinese healthy male volunteers by HPLC tandem mass spectrometry.
pubmed:affiliation
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China.
pubmed:publicationType
Journal Article