Linked Life Data

16115680

Source:http://linkedlifedata.com/resource/pubmed/id/16115680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-10-21
pubmed:abstractText
Temperature-responsive polymers are attractive candidates for applications related to injectable delivery of biologically active therapeutics, such as stem cells. In this study, we evaluate the potential of thermosensitive hydroxybutyl chitosan (HBC) as a biomaterial for the culture of human mesenchymal stem cells (hMSC) and cells derived from the intervertebral disk, with the eventual goal of using the HBC polymer as an injectable matrix/cell therapeutic. Conjugation of hydroxybutyl groups to chitosan renders the polymer water soluble and thermally responsive. Below its lower critical solution temperature, a solution of HBC can be maintained indefinitely in its solvated state. Upon exposure to a 37 degrees C environment, within 60 s, a 3.8 wt% HBC solution rapidly forms a gel that can be maneuvered with forceps. Upon cooling, the gel once again is able to revert to its solvated state. The gel exhibits a dramatic increase in both G' and G'' with increasing temperature, signifying a temperature-dependent enhancement of gel mechanical properties. Although a solid structure upon gelation, due to its physical nature of polymer interaction and gel formation, the gel exhibits a fluid-like viscoelastic behavior when exposed to shear stresses of up to 10% strain, with both G' and G'' approaching zero with increasing shear stress. Formulations of HBC gels presented in this study have gelation temperatures ranging from 13.0 to 34.6 degrees C and water contents of 67-95%. Minimal cytotoxicity in MSC and disk cell cultures was observed with these polymers up to a concentration of 5 wt%. Detection of metabolic activity, genetic analysis of synthesized mRNA, and histological staining of MSC and disk cell cultures in these gels collectively indicate cell proliferation without a loss in metabolic activity and extracellular matrix production. This study suggests the potential of HBC gel as an injectable carrier for future applications of delivering therapeutics to encourage a biologically relevant reconstruction of the degenerated disk.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
406-18
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16115680-Aggrecans, pubmed-meshheading:16115680-Butanols, pubmed-meshheading:16115680-Cell Proliferation, pubmed-meshheading:16115680-Cell Survival, pubmed-meshheading:16115680-Chitosan, pubmed-meshheading:16115680-Chondrocytes, pubmed-meshheading:16115680-Collagen, pubmed-meshheading:16115680-Collagen Type I, pubmed-meshheading:16115680-Extracellular Matrix Proteins, pubmed-meshheading:16115680-Gels, pubmed-meshheading:16115680-Gene Expression, pubmed-meshheading:16115680-Humans, pubmed-meshheading:16115680-Intervertebral Disc, pubmed-meshheading:16115680-Lectins, C-Type, pubmed-meshheading:16115680-Mesenchymal Stem Cells, pubmed-meshheading:16115680-Molecular Weight, pubmed-meshheading:16115680-Proteoglycans, pubmed-meshheading:16115680-Rheology, pubmed-meshheading:16115680-Tissue Culture Techniques, pubmed-meshheading:16115680-Transition Temperature, pubmed-meshheading:16115680-Water
pubmed:year
2006
pubmed:articleTitle
Temperature-responsive hydroxybutyl chitosan for the culture of mesenchymal stem cells and intervertebral disk cells.
pubmed:affiliation
Department of Biomedical Engineering, Johns Hopkins University, 720 Rutland Avenue, Ross 729, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article