Linked Life Data

16115199

Source:http://linkedlifedata.com/resource/pubmed/id/16115199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-8-23
pubmed:abstractText
Cra (or FruR), a global transcription factor with both repression and activation activities, controls a large number of the genes for glycolysis and gluconeogenesis. To get insights into the entire network of transcription regulation of the E. coli genome by Cra, we isolated a set of Cra-binding sequences using an improved method of genomic SELEX. From the DNA sequences of 97 independently isolated DNA fragments by SELEX, the Cra-binding sequences were identified in a total of ten regions on the E. coli genome, including promoters of six known genes and four hitherto-unidentified genes. All six known promoters are repressed by Cra, but none of the activation-type promoters were cloned after two cyles of SELEX, because the Cra-binding affinity to the repression-type promoters is higher than the activation-type promoters, as determined by the quantitative gel shift assay. Of a total of four newly identified Cra-binding sequences, two are associated with promoter regions of the gapA (glyceraldehyde 3-phosphate dehydrogenase) and eno (enolase) genes, both involved in sugar metabolism. The regulation of newly identified genes by Cra was confirmed by the in vivo promoter strength assay using a newly developed TFP (two-fluorescent protein) vector for promoter assay or by in vitro transcription assay in the presence of Cra protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1356-9597
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
907-18
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16115199-Bacterial Proteins, pubmed-meshheading:16115199-Base Sequence, pubmed-meshheading:16115199-Consensus Sequence, pubmed-meshheading:16115199-Dose-Response Relationship, Drug, pubmed-meshheading:16115199-Electrophoretic Mobility Shift Assay, pubmed-meshheading:16115199-Escherichia coli Proteins, pubmed-meshheading:16115199-Gene Expression Regulation, Bacterial, pubmed-meshheading:16115199-Genome, Bacterial, pubmed-meshheading:16115199-Genomic Library, pubmed-meshheading:16115199-Genomics, pubmed-meshheading:16115199-Glyceraldehyde-3-Phosphate Dehydrogenases, pubmed-meshheading:16115199-Molecular Sequence Data, pubmed-meshheading:16115199-Open Reading Frames, pubmed-meshheading:16115199-Phosphopyruvate Hydratase, pubmed-meshheading:16115199-Promoter Regions, Genetic, pubmed-meshheading:16115199-Repressor Proteins, pubmed-meshheading:16115199-Transcription, Genetic
pubmed:year
2005
pubmed:articleTitle
Systematic search for the Cra-binding promoters using genomic SELEX system.
pubmed:affiliation
Nippon Institute for Biological Science, Division of Molecular Biology, Ome, Tokyo 198-0024, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't