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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-8-23
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pubmed:abstractText |
The LBP-1 family consists of four proteins, which act as transcription factors in the formation of dimers with a member of this family. LBP-1a and LBP-1b are splicing variants from one gene, and LBP-1c and LBP-1d also arise from the alternative splicing of another gene. Investigation of subcellular localization of LBP-1 proteins fused to YFP revealed that the LBP-1b was localized in the nucleus, whereas LBP-1a and LBP-1c were exclusively localized in the cytosol. The peptide of 36 amino acids encoded by exon 6, a specific exon used only for LBP-1b, possessed the function of a nuclear localization signal (NLS). Nuclear localization of LBP-1a and LBP-1c occurred when LBP-1b was co-expressed, suggesting that heterodimerization of LBP-1a and LBP-1c with LBP-1b is important for their nuclear transport. Transiently expressed LBP-1 proteins in COS-7 cells formed speckles in the nucleus. Most speckles overlapped with the PML body. The activity of LBP-1a for accumulation in the PML body was mapped in the N-terminal region.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1356-9597
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
861-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16115195-Active Transport, Cell Nucleus,
pubmed-meshheading:16115195-Alternative Splicing,
pubmed-meshheading:16115195-Amino Acid Sequence,
pubmed-meshheading:16115195-Animals,
pubmed-meshheading:16115195-Bacterial Proteins,
pubmed-meshheading:16115195-COS Cells,
pubmed-meshheading:16115195-Cell Nucleus,
pubmed-meshheading:16115195-Cercopithecus aethiops,
pubmed-meshheading:16115195-DNA-Binding Proteins,
pubmed-meshheading:16115195-Dimerization,
pubmed-meshheading:16115195-HeLa Cells,
pubmed-meshheading:16115195-Humans,
pubmed-meshheading:16115195-Luminescent Proteins,
pubmed-meshheading:16115195-Microscopy, Fluorescence,
pubmed-meshheading:16115195-Molecular Sequence Data,
pubmed-meshheading:16115195-Nuclear Localization Signals,
pubmed-meshheading:16115195-Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Heterodimerization with LBP-1b is necessary for nuclear localization of LBP-1a and LBP-1c.
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pubmed:affiliation |
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai 980-8578, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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