Linked Life Data

1610962

Source:http://linkedlifedata.com/resource/pubmed/id/1610962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-7-30
pubmed:abstractText
Synthetic fluorescent substrates for thrombin were immobilized on a polyurethane membrane. The interaction of thrombin with the immobilized substrates was investigated. Upon hydrolysis of immobilized substrates by thrombin, fluorescence was emitted, intensity increasing linearly with time. From a Lineweaver-Burk plot, kinetic parameters of the enzyme reaction on the membrane surface were determined. The dissociation constant (K) was increased and the catalytic constant (k) decreased markedly by immobilization. More reactive thrombin substrates in solution were also more reactive in the immobilized state. More thrombin was adsorbed and adsorbed thrombins were inactivated more strongly on the surface with more reactive substrates when immobilized. The immobilization of thrombin substrate markedly suppressed fibrin formation on the membrane surface and made the membrane non-thrombogenic.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Interaction of thrombin with synthetic fluorescent substrate immobilized on polymer membrane.
pubmed:affiliation
Department of Polymer Chemistry, Kyoto University, Japan.
pubmed:publicationType
Journal Article, In Vitro