Linked Life Data

16107267

Source:http://linkedlifedata.com/resource/pubmed/id/16107267

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-10-31
pubmed:abstractText
To determine critical role of cyclooxygenase-2 (COX-2) for development of viral myocarditis, a mouse model of encephalomyocarditis virus-induced myocarditis was used. The virus was intraperitoneally given to COX-2 gene-deficient heterozygote mice (COX-2+/-) and wild-type mice (WT). We examined differences in heart weights, cardiac histological scores, numbers of infiltrating or apoptotic cells in myocardium, cardiac expression levels of COX-2, tumor necrosis factor-alpha (TNF-alpha), and adiponectin mRNA, immunoreactivity of COX-2, TNF-alpha, and adiponectin in myocytes, cardiac concentrations of TNF-alpha and adiponectin, prostaglandin E2 (PGE2) levels in hearts, and viral titers in tissues between COX-2+/- and WT. We observed significantly decreased expression of COX-2 mRNA and reactivity in hearts from COX-2+/- on day 8 after viral inoculation as compared with that from WT, together with elevated cardiac weights and severe inflammatory myocardial damage in COX-2+/-. Cardiac expression of TNF-alpha mRNA, reactivity, and protein on day 8 was significantly higher in COX-2+/- than in WT, together with reciprocal expression of adiponectin mRNA, reactivity, and protein in hearts. Significantly reduced cardiac PGE2 levels on day 8 were found in COX-2+/- compared with those in WT. There was no difference in local viral titers between both groups on day 4. Infected WT treated with a selective COX-2 inhibitor, NS-398, also showed the augmented myocardial damage on day 8. These results suggest that inhibition of COX-2 may enhance myocardial damage through reciprocal cardiac expression of TNF-alpha and adiponectin in a mouse model of viral myocarditis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-204
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16107267-Adiponectin, pubmed-meshheading:16107267-Animals, pubmed-meshheading:16107267-Body Weight, pubmed-meshheading:16107267-Cardiovirus Infections, pubmed-meshheading:16107267-Cyclooxygenase 2, pubmed-meshheading:16107267-Cyclooxygenase 2 Inhibitors, pubmed-meshheading:16107267-Dinoprostone, pubmed-meshheading:16107267-Encephalomyocarditis virus, pubmed-meshheading:16107267-Mice, pubmed-meshheading:16107267-Mice, Inbred C57BL, pubmed-meshheading:16107267-Mice, Knockout, pubmed-meshheading:16107267-Myocarditis, pubmed-meshheading:16107267-Myocardium, pubmed-meshheading:16107267-Myocytes, Cardiac, pubmed-meshheading:16107267-Nitrobenzenes, pubmed-meshheading:16107267-Organ Size, pubmed-meshheading:16107267-RNA, Messenger, pubmed-meshheading:16107267-Sulfonamides, pubmed-meshheading:16107267-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Inhibition of cyclooxygenase-2 enhances myocardial damage in a mouse model of viral myocarditis.
pubmed:affiliation
Department of a General Medicine, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't