Linked Life Data

16106218

Source:http://linkedlifedata.com/resource/pubmed/id/16106218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2005-8-17
pubmed:abstractText
Sphingosine-1-phosphate is a bioactive lipid that is mitogenic for human glioma cell lines by signaling through its G protein-coupled receptors. We investigated the role of sphingosine-1-phosphate receptors and the enzymes that form sphingosine-1-phosphate, sphingosine kinase (SphK)-1, and -2 in human astrocytomas. Astrocytomas of various histologic grades expressed three types of sphingosine-1-phosphate receptors, S1P1, S1P2, and S1P3; however, no significant correlation with histologic grade or patient survival was detected. Expression of SphK1, but not SphK2, in human astrocytoma grade 4 (glioblastoma multiforme) tissue correlated with short patient survival. Patients whose tumors had low SphK1 expression survived a median 357 days, whereas those with high levels of SphK1 survived a median 102 days. Decreasing SphK1 expression using RNA interference or pharmacologic inhibition of SphK significantly decreased the rate of proliferation of U-1242 MG and U-87 MG glioblastoma cell lines. Surprisingly, RNA interference to knockdown SphK2 expression inhibited glioblastoma cell proliferation more potently than did SphK1 knockdown. SphK knockdown also prevented cells from exiting G1 phase of the cell cycle and marginally increased apoptosis. Thus, SphK isoforms may be major contributors to growth of glioblastoma cells in vitro and to aggressive behavior of glioblastoma multiforme.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3069
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
695-705
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16106218-Adult, pubmed-meshheading:16106218-Apoptosis, pubmed-meshheading:16106218-Blotting, Northern, pubmed-meshheading:16106218-Blotting, Western, pubmed-meshheading:16106218-Cell Count, pubmed-meshheading:16106218-Cell Cycle, pubmed-meshheading:16106218-Cell Enlargement, pubmed-meshheading:16106218-Cell Line, Tumor, pubmed-meshheading:16106218-Gene Expression Regulation, Neoplastic, pubmed-meshheading:16106218-Glioblastoma, pubmed-meshheading:16106218-Humans, pubmed-meshheading:16106218-Middle Aged, pubmed-meshheading:16106218-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:16106218-RNA, Messenger, pubmed-meshheading:16106218-RNA, Small Interfering, pubmed-meshheading:16106218-Receptors, Lysosphingolipid, pubmed-meshheading:16106218-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16106218-Time Factors
pubmed:year
2005
pubmed:articleTitle
Sphingosine kinase-1 expression correlates with poor survival of patients with glioblastoma multiforme: roles of sphingosine kinase isoforms in growth of glioblastoma cell lines.
pubmed:affiliation
Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA. van-brocklyn.1@osu.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural