|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
13
|
|
pubmed:dateCreated |
2005-12-5
|
|
pubmed:abstractText |
Platelet activation in response to dual stimulation with collagen and thrombin results in the formation of a subpopulation of activated platelets known as coated platelets. Coated platelets are characterized by high surface levels of alpha-granule proteins and phosphatidylserine, which support the assembly of procoagulant protein complexes. Using murine models, we tested the hypothesis that the collagen receptor-associated molecule FcRgamma and the transglutaminase factor XIIIA are required for the formation of coated platelets. Following dual stimulation with the collagen receptor agonist convulxin and thrombin, 68% of platelets from C57BL/6 mice acquired the coated platelet phenotype, defined by high surface levels of fibrinogen and von Willebrand factor and decreased binding of the alphaIIbbeta3 activation-dependent antibody PE-JON/A. In FcRgamma-/- mice, only 10% of platelets became "coated" after dual stimulation with convulxin plus thrombin (P < .05 vs C57BL/6 platelets). Decreased coated platelet formation in FcRgamma-/- platelets was accompanied by decreased annexin V binding (P < .01) and decreased platelet procoagulant activity (P < .05). Platelets from FXIIIA-/- mice did not differ from control platelets in coated platelet formation or annexin V binding. We conclude that FcRgamma, but not factor XIIIA, is essential for formation of highly procoagulant coated platelets following dual stimulation with collagen and thrombin.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-10688826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-10825177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-10958788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-11113189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-11753286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-11805836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12116368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12351391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12351393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12529747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12861374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12871297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12913789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-12933578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-14697203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-15131115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-15507206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-15653564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-2866189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-8313472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16105983-9326228
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
106
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4146-51
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16105983-Animals,
pubmed-meshheading:16105983-Annexin A5,
pubmed-meshheading:16105983-Blood Platelets,
pubmed-meshheading:16105983-Cattle,
pubmed-meshheading:16105983-Cell Differentiation,
pubmed-meshheading:16105983-Factor XIIIa,
pubmed-meshheading:16105983-Mice,
pubmed-meshheading:16105983-Mice, Inbred C57BL,
pubmed-meshheading:16105983-P-Selectin,
pubmed-meshheading:16105983-Protein Binding,
pubmed-meshheading:16105983-Receptors, IgG,
pubmed-meshheading:16105983-Time Factors
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Role of FcRgamma and factor XIIIA in coated platelet formation.
|
|
pubmed:affiliation |
Department of Pediatrics, University of Iowa College of Medicine, Iowa City, IA 52242, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
