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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-1-13
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pubmed:abstractText |
Phosphodiesterase (PDE) 3 has been characterized in isolated rat submandibular acini. PDE3 activity was detected in homogenates of isolated rat submandibular acini; little or no PDE3 activity was found in ducts. About 62% of PDE3 activity in the acini was recovered in the supernatant fractions; 38% in particulate fractions. In the acini, but not ducts, PDE3A mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The PDE3-specific inhibitor, cilostamide, increased the ratio of apomucin mRNA/18s rRNA, as quantified by real-time RT-PCR. Our results indicate that PDE3A may be important in regulating cAMP pools that control acini functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0003-9969
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
83-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16102722-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:16102722-Animals,
pubmed-meshheading:16102722-Cells, Cultured,
pubmed-meshheading:16102722-Cyclic Nucleotide Phosphodiesterases, Type 3,
pubmed-meshheading:16102722-Gene Expression,
pubmed-meshheading:16102722-Male,
pubmed-meshheading:16102722-Phosphodiesterase Inhibitors,
pubmed-meshheading:16102722-Quinolones,
pubmed-meshheading:16102722-RNA, Messenger,
pubmed-meshheading:16102722-Rats,
pubmed-meshheading:16102722-Rats, Wistar,
pubmed-meshheading:16102722-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16102722-Submandibular Gland
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pubmed:year |
2006
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pubmed:articleTitle |
Expression of cyclic nucleotide phosphodiesterase 3A in isolated rat submandibular acini.
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pubmed:affiliation |
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. skasumi@clin.medic.mie-u.ac.jp
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pubmed:publicationType |
Journal Article
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