Linked Life Data

16102421

Source:http://linkedlifedata.com/resource/pubmed/id/16102421

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-8-16
pubmed:abstractText
Three full-length infectious cDNA clones based on the alphavirus chikungunya (CHIKV) were developed and characterized in vitro and in vivo. The full-length clone retained the viral phenotypes of CHIKV in both cell culture and in mosquitoes and should be a valuable tool for the study of virus interactions in an epidemiologically significant natural vector, Aedes aegypti. Two additional infectious clones were constructed that express green fluorescent protein (EGFP) in the midgut, salivary glands, and nervous tissue of Aedes aegypti mosquitoes following oral infection. The two constructs differed in the placement of the subgenomic promoter and the gene encoding EGFP. Viruses derived from the pCHIKic EGFP constructs (5' CHIKV EGFP and 3' CHIKV EGFP) expressed EGFP in 100% of the Ae. aegypti mosquitoes tested on days 7 and 14 post infection (p.i.). The 5' CHIKV EGFP disseminated to 90% of the salivary glands and nervous tissue by day 14 p.i. Dissemination rates of this new viral vector exceeds those of previous systems, thus expanding the repertoire and potential for gene expression studies on this important vector species.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0965-1748
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1162-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in Aedes aegypti mosquitoes.
pubmed:affiliation
Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural