16101486

Source:http://linkedlifedata.com/resource/pubmed/id/16101486

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0001128, umls-concept:C0011945, umls-concept:C0079809, umls-concept:C0242485, umls-concept:C0678594, umls-concept:C0679932, umls-concept:C1510438, umls-concept:C1704675
pubmed:issue	4
pubmed:dateCreated	2005-8-16
pubmed:abstractText	Competition dialysis is a powerful new tool for the discovery of ligands that bind to nucleic acids with structural- or sequence-selectivity. The method is based on firm thermodynamic principles and is simple to implement. In the competition dialysis experiment, an array of nucleic acid structures and sequences is dialyzed against a common test ligand solution. After equilibration, the amount of ligand bound to each structure or sequence is determined spectrophotometrically. Since all structures and sequences are in equilibrium with the same free ligand concentration, the amount bound is directly proportional to the ligand binding affinity. Competition dialysis thus provides a direct and quantitative measure of selectivity, and unambiguously identifies which of the structures or sequences within the sample array that are preferred by a particular ligand. Following the introduction of the method, competition dialysis has been used worldwide to probe a variety of ligand-nucleic acid interactions. This contribution will focus on new analytical approaches for extracting information from the database that resulted from the first-generation competition dialysis assay, in which binding data was gathered for the interaction of 126 compounds with 13 different structures and sequences. Such global analyses allow identification of compounds with unique types of binding selectivity.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/CA35635
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101123597
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acids
pubmed:status	MEDLINE
pubmed:month	Jul
pubmed:issn	1568-0118
pubmed:author	pubmed-author:ChairesJonathan BJB
pubmed:issnType	Print
pubmed:volume	5
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	339-52
pubmed:dateRevised	2007-11-14
pubmed:meshHeading	pubmed-meshheading:16101486-Binding, Competitive, pubmed-meshheading:16101486-Dialysis, pubmed-meshheading:16101486-Drug Interactions, pubmed-meshheading:16101486-Nucleic Acid Conformation, pubmed-meshheading:16101486-Nucleic Acids
pubmed:year	2005
pubmed:articleTitle	Competition dialysis: an assay to measure the structural selectivity of drug-nucleic acid interactions.
pubmed:affiliation	James Graham Brown Cancer Center, Department of Medicine, Health Sciences Center, University of Louisville, 529 South Jackson Street, Louisville, KY 40202, USA. j.chaires@louisville.edu
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural