Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Suppl 1
pubmed:dateCreated
2005-8-16
pubmed:abstractText
Melanoma cells exhibit a high level of intrinsic or acquired resistance to the cytotoxic agents often associated with the over-expression of drug transporters such as P-glycoprotein (P-gp). In this in vitro study, we investigated the possible relationship between P-gp and CD44, the cell adhesion molecule involved in metastasis and tumor progression of melanoma cells. CD44 expression appeared to be similar in the parental sensitive M14 WT cells and in their resistant counterparts M14 ADR cells. Double-labeling of cryosectioned cells showed that P-gp and CD44 were transported from the synthesis loci to the cell periphery by different vesicles and began to coalesce in proximity of the plasma membrane; thus, P-gp and CD44 seemed to reach together the cell surface. Moreover, P-gp and CD44 appeared to be associated with ERM proteins. The invasive activities of both M14 WT and M14 ADR cells were analyzed by the "transwell chamber invasion" assay. M14 WT cells revealed low capacity to traverse the filters, both in the absence (motility) and in the presence (invasion) of a Matrigel coating. In comparison, M14 ADR cells displayed significantly higher motility and invasion. SEM observations showed that sensitive cells employed lamellar cytoplasmic extrusions to pass through the filter pores whereas resistant cells elongated along the hole through globular processes. In conclusion, the results herein reported suggest that drug resistance in melanoma cells appears associated with a more aggressive behaviour. P-gp and CD44 might cooperate to confer this more invasive phenotype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1122-6714
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-41
pubmed:meshHeading
pubmed-meshheading:16101031-Antigens, CD44, pubmed-meshheading:16101031-Carrier Proteins, pubmed-meshheading:16101031-Cell Adhesion Molecules, pubmed-meshheading:16101031-Cell Line, Tumor, pubmed-meshheading:16101031-Cell Membrane, pubmed-meshheading:16101031-Collagen, pubmed-meshheading:16101031-Drug Combinations, pubmed-meshheading:16101031-Drug Resistance, Multiple, pubmed-meshheading:16101031-Drug Resistance, Neoplasm, pubmed-meshheading:16101031-Humans, pubmed-meshheading:16101031-Laminin, pubmed-meshheading:16101031-Macromolecular Substances, pubmed-meshheading:16101031-Melanoma, pubmed-meshheading:16101031-Microscopy, Electron, Scanning, pubmed-meshheading:16101031-Microscopy, Electron, Transmission, pubmed-meshheading:16101031-Neoplasm Invasiveness, pubmed-meshheading:16101031-P-Glycoprotein, pubmed-meshheading:16101031-Phenotype, pubmed-meshheading:16101031-Protein Transport, pubmed-meshheading:16101031-Proteoglycans, pubmed-meshheading:16101031-Pseudopodia
pubmed:year
2005
pubmed:articleTitle
Invasive properties of multidrug resistant human melanoma cells.
pubmed:affiliation
Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy.
pubmed:publicationType
Journal Article