Linked Life Data

16099927

Source:http://linkedlifedata.com/resource/pubmed/id/16099927

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-11-18
pubmed:abstractText
Previous studies have reported the sex differences in heart susceptibility to ischemia/reperfusion (I/R) injury, but the mechanisms are not understood. The present study tested the hypothesis that Akt and protein kinase C (PKC)epsilon play an important role in the sexual dimorphism of heart susceptibility to I/R injury. Isolated hearts from 2-month-old male and female rats were subjected to I/R in the Langendorff preparation. The postischemic recovery of left ventricular function was significantly better, and infarct size was significantly smaller in female (37.1 +/- 1.9%) than in male (48.3 +/- 2.3%) hearts after 25-min ischemia followed by 2-h reperfusion. Inhibition of phosphatidylinositol 3-kinase/Akt pathway by wortmannin or PKC by chelerythrine chloride before ischemia significantly reduced postischemic recovery and increased infarct size in female but not male hearts. There were no differences in myocardial protein levels of heat shock protein 70, Akt, and PKCepsilon, respectively, between male and female rats. However, the ratio of phosphorylated (p)-Akt/Akt (0.58 +/- 0.05 versus 0.22 +/- 0.04; P < 0.05) and p-PKCepsilon/PKCepsilon (0.35 +/- 0.03 versus 0.22 +/- 0.02; P < 0.05) was significantly higher in female than in male hearts. In addition, there were significant increases in p-Akt and p-PKCepsilon levels during reperfusion in female but not in male hearts. The results suggest that increased p-Akt and p-PKCepsilon levels in female hearts contribute to the gender-related differences in heart susceptibility to I/R and play an important role in cardioprotection against I/R injury in females.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
315
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1125-35
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16099927-Alkaloids, pubmed-meshheading:16099927-Androstadienes, pubmed-meshheading:16099927-Animals, pubmed-meshheading:16099927-Benzophenanthridines, pubmed-meshheading:16099927-Blotting, Western, pubmed-meshheading:16099927-Enzyme Activation, pubmed-meshheading:16099927-Female, pubmed-meshheading:16099927-HSP70 Heat-Shock Proteins, pubmed-meshheading:16099927-Heart Ventricles, pubmed-meshheading:16099927-Male, pubmed-meshheading:16099927-Myocardial Infarction, pubmed-meshheading:16099927-Myocardial Ischemia, pubmed-meshheading:16099927-Myocardial Reperfusion Injury, pubmed-meshheading:16099927-Phenanthridines, pubmed-meshheading:16099927-Phosphatidylinositol 3-Kinases, pubmed-meshheading:16099927-Protein Kinase C, pubmed-meshheading:16099927-Proto-Oncogene Proteins c-akt, pubmed-meshheading:16099927-Rats, pubmed-meshheading:16099927-Rats, Sprague-Dawley, pubmed-meshheading:16099927-Sex Factors, pubmed-meshheading:16099927-Signal Transduction, pubmed-meshheading:16099927-Time Factors, pubmed-meshheading:16099927-Ventricular Dysfunction, Left
pubmed:year
2005
pubmed:articleTitle
Gender differences in cardioprotection against ischemia/reperfusion injury in adult rat hearts: focus on Akt and protein kinase C signaling.
pubmed:affiliation
Center for Perinatal Biology, Department of Pharmacology and Physiology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural