Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-8-15
pubmed:abstractText
Tumor-stroma interactions play a decisive role in the growth and metastasis of solid tumors, and involve signalling either by soluble mediators or direct cell-cell interaction. Here, we report the isolation and characterisation of a novel cDNA (MEL4B3), which is induced in cultured dermal fibroblasts exposed to supernatants of melanoma cell lines. MEL4B3 shares high homology with two predicted cDNA sequences for which no activity has so far been described. In situ hybridisation revealed the expression of MEL4B3 in malignant melanoma increasing with tumor depth; in basal cell carcinoma and in squamous cell carcinoma. MEL4B3 was barely detectable in normal skin or non-malignant melanocytic naevi. Furthermore, MEL4B3 was expressed at high level in the epidermis of psoriatic skin. In vitro, the expression of MEL4B3 was found to be induced by the exposure of human dermal fibroblasts to melanoma cell culture supernatants or to transforming growth factor-beta, interleukin-1 and tumor necrosis factor-alpha. The expression MEL4B3 therefore reflects closely cell activation occurring during tumor growth, metastasis and inflammation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0906-6705
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
709-18
pubmed:dateRevised
2007-6-9
pubmed:meshHeading
pubmed-meshheading:16098131-Amino Acid Sequence, pubmed-meshheading:16098131-Base Sequence, pubmed-meshheading:16098131-Blotting, Northern, pubmed-meshheading:16098131-Carcinoma, Basal Cell, pubmed-meshheading:16098131-Carcinoma, Squamous Cell, pubmed-meshheading:16098131-Cells, Cultured, pubmed-meshheading:16098131-Culture Media, pubmed-meshheading:16098131-Culture Media, Conditioned, pubmed-meshheading:16098131-Cytokines, pubmed-meshheading:16098131-DNA, Complementary, pubmed-meshheading:16098131-Fibroblasts, pubmed-meshheading:16098131-Flow Cytometry, pubmed-meshheading:16098131-Gene Expression Regulation, Neoplastic, pubmed-meshheading:16098131-Humans, pubmed-meshheading:16098131-In Situ Hybridization, pubmed-meshheading:16098131-Inflammation, pubmed-meshheading:16098131-Keratinocytes, pubmed-meshheading:16098131-Microcirculation, pubmed-meshheading:16098131-Molecular Sequence Data, pubmed-meshheading:16098131-Neoplasm Metastasis, pubmed-meshheading:16098131-Neoplasm Proteins, pubmed-meshheading:16098131-Polymerase Chain Reaction, pubmed-meshheading:16098131-RNA, Complementary, pubmed-meshheading:16098131-RNA, Messenger, pubmed-meshheading:16098131-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16098131-Skin, pubmed-meshheading:16098131-Skin Neoplasms, pubmed-meshheading:16098131-Transforming Growth Factor beta
pubmed:year
2005
pubmed:articleTitle
MEL4B3, a novel mRNA is induced in skin tumors and regulated by TGF-beta and pro-inflammatory cytokines.
pubmed:affiliation
Saxon Academy of Science in Leipzig, University of Leipzig, Leipzig, Germany. andu@medizin.uni-leipzig.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't