Source:http://linkedlifedata.com/resource/pubmed/id/16095973
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2005-11-21
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| pubmed:abstractText |
HIV-1 Nef incorporates into virions at low levels, likely about 10 molecules per viral particle. Here, we describe a Nef mutant (Nef7) apparently showing more than 100-fold higher efficiency of virion incorporation. Interestingly, Nef7 can act as a cargo molecule for protein delivery into the cells, as its virion incorporation appeared conserved even upon C-terminal fusion with proteins of up to 30 kDa. This was demonstrated first by assessing the intracellular fluorescence of cells challenged with lentivirus-based virus-like particles (VLPs) pseudotyped with the vesicular stomatitis virus envelope glycoprotein (VSV-G) and incorporating Nef7 fused with the green fluorescent protein. Furthermore, the biologic activity of products delivered by Nef7-based VLPs was demonstrated by tagging Nef7 with the herpes simplex virus-1 thymidine kinase (HSV-1 TK). In fact, we observed that both cell lines and primary human macrophages challenged with (VSV-G) Nef7/TK VLPs died after 5 to 7 days of treatment with ganciclovir (GCV). In sum, our findings support the notion that Nef7-based VLPs can be considered platforms for original systems of protein delivery. In particular, the here- described Nef7/TK VLPs represent a first applicative example opening the way toward new HSV-1 TK/GCV-based cell suicide therapies circumventing cell gene engineering procedures.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/G protein, vesicular stomatitis...,
http://linkedlifedata.com/resource/pubmed/chemical/Ganciclovir,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1525-0016
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1185-96
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16095973-Blotting, Western,
pubmed-meshheading:16095973-Cell Line,
pubmed-meshheading:16095973-Detergents,
pubmed-meshheading:16095973-Ganciclovir,
pubmed-meshheading:16095973-Gene Therapy,
pubmed-meshheading:16095973-Gene Transfer Techniques,
pubmed-meshheading:16095973-Green Fluorescent Proteins,
pubmed-meshheading:16095973-HIV-1,
pubmed-meshheading:16095973-Herpesvirus 1, Human,
pubmed-meshheading:16095973-Humans,
pubmed-meshheading:16095973-Kinetics,
pubmed-meshheading:16095973-Lentivirus,
pubmed-meshheading:16095973-Macrophages,
pubmed-meshheading:16095973-Membrane Glycoproteins,
pubmed-meshheading:16095973-Membrane Microdomains,
pubmed-meshheading:16095973-Models, Genetic,
pubmed-meshheading:16095973-Protein Structure, Tertiary,
pubmed-meshheading:16095973-Recombinant Fusion Proteins,
pubmed-meshheading:16095973-Retroviridae,
pubmed-meshheading:16095973-Thymidine Kinase,
pubmed-meshheading:16095973-Time Factors,
pubmed-meshheading:16095973-Viral Envelope Proteins
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| pubmed:year |
2005
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| pubmed:articleTitle |
Cell death induced by the herpes simplex virus-1 thymidine kinase delivered by human immunodeficiency virus-1-based virus-like particles.
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| pubmed:affiliation |
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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