Linked Life Data

16092086

Source:http://linkedlifedata.com/resource/pubmed/id/16092086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-9-5
pubmed:abstractText
The effect of feeding lead (50 mg kg(-1) body weight) daily for 7 days on the development of various brush border enzymes in the intestine has been studied. The activities of brush border sucrase (P < 0.001), lactase (P < 0.001), gamma-glutamyl transpeptidase (P < 0.05) and leucine aminopeptidase were reduced (P < 0.05), whereas the alkaline phosphatase level was augmented (P < 0.05) in lead fed rats compared with controls. Kinetic studies with sucrase revealed a low Vmax (0.224 in control and 0.160 units mg(-1) protein in lead exposed) with no change in Km (12.6-13.5 mM). Western blot analysis for alkaline phosphatase yielded intense staining of enzyme protein in lead fed rats compared with controls, however, the intensity of the antigen signal was reversed for sucrase under these conditions. These findings suggest that ingestion of lead may interfere with the crypt cell differentiation process thus affecting enzyme functions in the rat intestine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0260-437X
pubmed:author
pubmed:copyrightInfo
2005 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-4
pubmed:meshHeading
pubmed:articleTitle
Expression of brush border enzymes in response to lead exposure in rat intestine.
pubmed:affiliation
Department of Biochemistry, Panjab University, Chandigarh-160 014, India.
pubmed:publicationType
Journal Article