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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-8-10
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pubmed:databankReference |
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pubmed:abstractText |
The lectin from Agrocybe aegerita (AAL) has been found to possess potent tumor-suppressing function and tumor cell apoptosis-inducing activity. In this paper, we report the full sequence, the active expression of the gene encoding AAL at a high level and bioassay of the binding property with lactose, apoptosis-inducing activity and DNase activity of recombinant AAL (rAAL). The results reveal that AAL is a member of the galectin family and the dimeric form is the active unit for the functional performance. The rAAL showed comparable tumor cell apoptosis-inducing activity with the wild AAL but no DNase activity at all. The molecular characters revealed by this study are significant for the in-depth investigation of the functional mechanism of this interesting protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
145-50
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:16091588-Agaricales,
pubmed-meshheading:16091588-Amino Acid Sequence,
pubmed-meshheading:16091588-Animals,
pubmed-meshheading:16091588-Antineoplastic Agents,
pubmed-meshheading:16091588-Apoptosis,
pubmed-meshheading:16091588-Cloning, Molecular,
pubmed-meshheading:16091588-DNA, Complementary,
pubmed-meshheading:16091588-Deoxyribonucleases,
pubmed-meshheading:16091588-Galectins,
pubmed-meshheading:16091588-HeLa Cells,
pubmed-meshheading:16091588-Hemagglutination Tests,
pubmed-meshheading:16091588-Humans,
pubmed-meshheading:16091588-Lactose,
pubmed-meshheading:16091588-Mice,
pubmed-meshheading:16091588-Molecular Sequence Data,
pubmed-meshheading:16091588-Protein Binding,
pubmed-meshheading:16091588-Recombinant Proteins,
pubmed-meshheading:16091588-Structure-Activity Relationship
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pubmed:year |
2005
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pubmed:articleTitle |
Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita.
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pubmed:affiliation |
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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