Source:http://linkedlifedata.com/resource/pubmed/id/16086153
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2005-11-30
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| pubmed:abstractText |
Lysyl oxidase (LOX), an extracellular enzyme, plays a key role in the post-translational modification of collagens and elastin, catalyzing inter- and intra-crosslinking reactions. Because the crosslinked extracellular matrices (ECMs) are highly resistant to degradative enzymes, it is considered that the over-expression of LOX may cause severe fibrotic degeneration. In the present study, we addressed the role of LOX-mediated crosslinking in chronic renal tubulointerstitial fibrosis using an animal model of hereditary nephrotic syndrome, the Institute of Cancer Research (ICR)-derived glomerulonephritis (ICGN) mouse. Ribonuclease protection assay (RPA) revealed that LOX mRNA expression was up-regulated in the kidneys of ICGN mice as compared with control ICR mice. High-level expression of LOX and transforming growth factor (TGF)-beta1 (an up-regulator of LOX) mRNA was detected in tubular epithelial cells of ICGN mouse kidneys by in situ hybridization. Type-I and -III collagens, major substrates for LOX, were accumulated in tubulointerstitium of ICGN mouse kidneys. The present findings imply that TGF-beta1 up-regulates the production of LOX in tubular epithelial cells of ICGN mouse kidneys, and the excessive LOX acts on interstitial collagens and catalyzes crosslinking reactions. As a result, the highly crosslinked collagens induce an irreversible progression of chronic renal tubulointerstitial fibrosis in the kidneys of ICGN mice.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Lysine 6-Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
|
| pubmed:issn |
0945-6317
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
447
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
859-68
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16086153-Animals,
pubmed-meshheading:16086153-Cells, Cultured,
pubmed-meshheading:16086153-Chronic Disease,
pubmed-meshheading:16086153-Disease Models, Animal,
pubmed-meshheading:16086153-Dose-Response Relationship, Drug,
pubmed-meshheading:16086153-Epithelial Cells,
pubmed-meshheading:16086153-Female,
pubmed-meshheading:16086153-Fibrosis,
pubmed-meshheading:16086153-Glomerulonephritis,
pubmed-meshheading:16086153-Humans,
pubmed-meshheading:16086153-In Situ Hybridization,
pubmed-meshheading:16086153-Kidney,
pubmed-meshheading:16086153-Kidney Tubules,
pubmed-meshheading:16086153-Male,
pubmed-meshheading:16086153-Mice,
pubmed-meshheading:16086153-Mice, Inbred ICR,
pubmed-meshheading:16086153-Nephrotic Syndrome,
pubmed-meshheading:16086153-Protein-Lysine 6-Oxidase,
pubmed-meshheading:16086153-RNA, Messenger,
pubmed-meshheading:16086153-Transforming Growth Factor beta,
pubmed-meshheading:16086153-Transforming Growth Factor beta1,
pubmed-meshheading:16086153-Up-Regulation
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| pubmed:year |
2005
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| pubmed:articleTitle |
Transforming growth factor-beta1 mediated up-regulation of lysyl oxidase in the kidneys of hereditary nephrotic mouse with chronic renal fibrosis.
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| pubmed:affiliation |
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo, 3145 Ago, Ibaraki-Iwama, 319-0206, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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