Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2005-8-8
pubmed:abstractText
A human papillomavirus (HPV) multiplexed competitive Luminex immunoassay first described by Opalka et al. (D. Opalka, C. E. Lachman, S. A. MacMullen, K. U. Jansen, J. F. Smith, N. Chirmule, and M. T. Esser, Clin. Diagn. Lab. Immunol. 10:108--15, 2003) was optimized and validated for use in epidemiology studies and vaccine clinical trials. Optimization increased both the analytical sensitivity and the clinical specificity of the assay to more effectively discriminate the low-titer antibody response of HPV-infected persons from noninfected individuals. The characteristics of the assay that were optimized included monoclonal antibody (MAb) specificity, scaling up the conjugation of virus-like particles (VLPs) to microspheres, VLP concentration, MAb concentration, sample matrix, sample dilution, incubation time, heat inactivation of sample sera, and detergent effects on assay buffer. The assay was automated by use of a TECAN Genesis Workstation, thus improving assay throughput, reproducibility, and operator safety. Following optimization, the assay was validated using several distinct serum panels from individuals determined to be at low and high risk for HPV infection. The validated assay was then used to determine the clinical serostatus cutoff. This high-throughput assay has proven useful for performing epidemiology studies and evaluating the efficacy of prophylactic HPV vaccines.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-10600468, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-10700419, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-10834618, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-10837170, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11280749, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11343204, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11602373, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11701422, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11902586, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11919208, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-11986263, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12404154, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12444178, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12522048, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12571259, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12867641, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-12953088, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-14746524, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-14982672, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-15028941, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-15478060, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-15541448, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-15604432, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-15863374, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-2170694, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-3528294, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-3740412, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-7526536, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-7778283, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-7791229, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-8601783, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-8806551, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-8874508, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-8973339, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-9292008, http://linkedlifedata.com/resource/pubmed/commentcorrection/16085914-9333144
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
959-69
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Optimization and validation of a multiplexed luminex assay to quantify antibodies to neutralizing epitopes on human papillomaviruses 6, 11, 16, and 18.
pubmed:affiliation
Vaccine and Biologics Research, Merck Research Laboratories, 466 Devon Park Dr., Wayne, PA 19087-8630, USA.
pubmed:publicationType
Journal Article, Validation Studies