|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2005-8-15
|
|
pubmed:abstractText |
Orexinergic and norepinephrinergic alpha2-adrenoceptor expressing neurons contribute to the regulation of the sleep-wakefulness cycle. In the present study, we have examined a possible interaction between orexinergic and alpha2-adrenergic systems in orexin-A (100 nM)- and K+ (25 mM)-evoked norepinephrine release from slices of rat cerebrocortex. In this tissue norepinephrinergic neurons are predominantly innervated via the locus coeruleus. Clonidine concentration-dependently inhibited K+-evoked norepinephrine release with pIC50 (Imax) of 6.44+/-0.38 (48.8+/-6.9%). A selective orexin-1 receptor antagonist, SB-334867 was ineffective. SB-334867 concentration-dependently inhibited orexin A-evoked norepinephrine release with pIC50 (Imax) of 6.05+/-0.14 (86.4+/-5.4%); clonidine (alpha2-agonist) was ineffective. In contrast, yohimbine reversed the inhibitory effects of clonidine (1 microM) on K+-evoked norepinephrine release with pIC50 (Imax) of 6.50+/-0.34 (77.6+/-10.9%); orexin A was ineffective. The present data suggest a lack of interaction between orexinergic and alpha2-adrenergic neurons in rat cerebral cortex.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(2-methylbenzoxazol-6-yl)-3-(1,5)n...,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-2 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Clonidine,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neuropeptide,
http://linkedlifedata.com/resource/pubmed/chemical/Urea,
http://linkedlifedata.com/resource/pubmed/chemical/Yohimbine,
http://linkedlifedata.com/resource/pubmed/chemical/orexin receptors,
http://linkedlifedata.com/resource/pubmed/chemical/orexins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0304-3940
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
387
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
49-52
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16085362-Adrenergic alpha-2 Receptor Agonists,
pubmed-meshheading:16085362-Adrenergic alpha-Agonists,
pubmed-meshheading:16085362-Adrenergic alpha-Antagonists,
pubmed-meshheading:16085362-Animals,
pubmed-meshheading:16085362-Benzoxazoles,
pubmed-meshheading:16085362-Cerebral Cortex,
pubmed-meshheading:16085362-Clonidine,
pubmed-meshheading:16085362-Dose-Response Relationship, Drug,
pubmed-meshheading:16085362-Drug Interactions,
pubmed-meshheading:16085362-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16085362-Locus Coeruleus,
pubmed-meshheading:16085362-Male,
pubmed-meshheading:16085362-Neural Pathways,
pubmed-meshheading:16085362-Neurons,
pubmed-meshheading:16085362-Neuropeptides,
pubmed-meshheading:16085362-Norepinephrine,
pubmed-meshheading:16085362-Organ Culture Techniques,
pubmed-meshheading:16085362-Potassium,
pubmed-meshheading:16085362-Rats,
pubmed-meshheading:16085362-Receptors, Adrenergic, alpha-2,
pubmed-meshheading:16085362-Receptors, G-Protein-Coupled,
pubmed-meshheading:16085362-Receptors, Neuropeptide,
pubmed-meshheading:16085362-Sleep,
pubmed-meshheading:16085362-Urea,
pubmed-meshheading:16085362-Wakefulness,
pubmed-meshheading:16085362-Yohimbine
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Lack of interaction between orexinergic and alpha2-adrenergic neuronal systems in rat cerebrocortical slices.
|
|
pubmed:affiliation |
Department of Anesthesiology, University of Hirosaki School of Medicine, Hirosaki 036-8563, Japan. masuika@cc.hirosaki-u.ac.jp
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
