Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-9-12
pubmed:abstractText
The in vitro effects of myotoxin III (MT-III), an Asp-49 catalytically-active phospholipase A(2), and myotoxin II (MT-II), a catalytically-inactive Lys-49 variant, isolated from Bothrops asper snake venom, on phagocytosis and production of hydrogen peroxide (H(2)O(2)) by thioglycollate-elicited macrophages were investigated. MT-II and MT-III were cytotoxic to mouse peritoneal macrophages at concentrations higher than 25 microg/ml. At non-cytotoxic concentrations, MT-II stimulated Fcgamma, complement, mannose and beta-glucan receptors-mediated phagocytosis, whereas MT-III stimulated only the mannose and beta-glucan receptors-mediated phagocytosis. Moreover, both myotoxins induced the release of H(2)O(2) by thioglycollate-elicited macrophages, MT-III being the most potent stimulator. MT-II induced the release of H(2)O(2) only at a concentration of 3.2 microg/ml (130% increment) while MT-III induced this effect at all concentrations tested (0.5-2.5 microg/ml; average of 206% increment). It is concluded that, at non-cytotoxic concentrations, MT-II and MT-III activate defense mechanisms in macrophages up regulating phagocytosis, mainly via mannose and beta-glucan receptors, and the respiratory burst.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Crotalid Venoms, http://linkedlifedata.com/resource/pubmed/chemical/Group II Phospholipases A2, http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fc Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents, http://linkedlifedata.com/resource/pubmed/chemical/Mannose, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Reptilian Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Zymosan, http://linkedlifedata.com/resource/pubmed/chemical/beta-glucan receptor, http://linkedlifedata.com/resource/pubmed/chemical/myotoxin II, Bothrops asper
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
523-32
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16085208-Animals, pubmed-meshheading:16085208-Bothrops, pubmed-meshheading:16085208-Candida albicans, pubmed-meshheading:16085208-Complement System Proteins, pubmed-meshheading:16085208-Crotalid Venoms, pubmed-meshheading:16085208-Group II Phospholipases A2, pubmed-meshheading:16085208-Hydrogen Peroxide, pubmed-meshheading:16085208-Immunoglobulin Fc Fragments, pubmed-meshheading:16085208-Indicators and Reagents, pubmed-meshheading:16085208-Macrophage Activation, pubmed-meshheading:16085208-Macrophages, pubmed-meshheading:16085208-Male, pubmed-meshheading:16085208-Mannose, pubmed-meshheading:16085208-Mice, pubmed-meshheading:16085208-Phagocytosis, pubmed-meshheading:16085208-Phospholipases A, pubmed-meshheading:16085208-Receptors, Immunologic, pubmed-meshheading:16085208-Reptilian Proteins, pubmed-meshheading:16085208-Sheep, pubmed-meshheading:16085208-Zymosan
pubmed:year
2005
pubmed:articleTitle
Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A(2).
pubmed:affiliation
Laboratorio de Farmacologia, Instituto Butantan, Av.Vital Brazil, 1500-CEP 05503-900 Sao Paulo, SP, Brazil. cteixer@usp.br
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't