Linked Life Data

16084209

Source:http://linkedlifedata.com/resource/pubmed/id/16084209

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-8-8
pubmed:abstractText
We present the first measurements of the tensile properties of embryonic epithelia, data that are crucial to understanding the mechanics of morphogenetic movements. Fine wires were glued to the surface of an intact, live embryo using cyanoacrylate glue, after which the epithelium between the wires was separated from the remainder of the embryo by microsurgery. The wires were then separated from each other in 0.1 microm steps under computer control in order to elongate the tissue at a constant true strain rate. Force was determined from the degree of bending in the wires, and a real-time, image-based feedback system corrected for reductions in elongation that would otherwise have been caused by wire flexure. The instrument was used to determine the tensile properties of epidermis and neuroepithelia from early-stage embryos of the axolotl (Ambystoma mexicanum), a type of amphibian. Monolayer specimens as small as 300 by 500 microm were elongated at physiological strain rates of 5-30% per hour, and the effects of developmental stage, epithelium type, specimen origin, direction of elongation and strain rate were investigated. True strains as high as 50% were observed before tearing began and equivalent moduli for the initial, linear portion of the load resultant versus strain curves ranged from 1 x 10(-3) to 8 x 10(-3) N/m.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9290
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2087-94
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Tensile properties of embryonic epithelia measured using a novel instrument.
pubmed:affiliation
Department of Civil Engineering, University of Waterloo, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't