Linked Life Data

16082727

Source:http://linkedlifedata.com/resource/pubmed/id/16082727

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-9-19
pubmed:abstractText
While acquisition of regulatory function by CD4+CD25- T cells has been reported following antigenic stimulation, "naturally occurring" regulatory CD4+ T cells (Treg) are believed to express CD25. We examined the mechanisms involved in peripheral CD8 T cell tolerance by induction of mixed chimerism using non-myeloablative conditioning with low-dose (3 Gy) total body irradiation and anti-CD154 antibody. Recipient CD4+ T cells were initially required for the induction of CD8 cell tolerance, but were not needed beyond 2 weeks. Depletion of CD25+ Treg prior to bone marrow transplantation and blockade of IL-2 with neutralizing antibody did not impede tolerance induction. Tolerance was dependent on CTLA4, but not on IFN-gamma. In C57BL/6 mice containing a fraction of 2C TCR transgenic CD8+ T cells, which recognize the MHC class I alloantigen Ld, induction of chimerism with L(d+), but not Ld-, bone marrow cells led to deletion of peripheral 2C+ CD8+ cells within 1 week in peripheral blood and spleen. Complete deletion required the presence of recipient CD4+ T cells. Thus, a novel, rapid form of regulation by CD4+CD25- T cells permits initial CD8 T cell tolerance in this model. Rapid peripheral deletion of donor-specific CD8 T cells precludes an ongoing requirement for CD4 T cell-mediated regulation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2679-90
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16082727-Animals, pubmed-meshheading:16082727-Antibodies, pubmed-meshheading:16082727-Antigens, CD, pubmed-meshheading:16082727-Antigens, Differentiation, pubmed-meshheading:16082727-Bone Marrow Transplantation, pubmed-meshheading:16082727-CD4-Positive T-Lymphocytes, pubmed-meshheading:16082727-CD40 Ligand, pubmed-meshheading:16082727-CD8-Positive T-Lymphocytes, pubmed-meshheading:16082727-CTLA-4 Antigen, pubmed-meshheading:16082727-Female, pubmed-meshheading:16082727-Graft Rejection, pubmed-meshheading:16082727-Immune Tolerance, pubmed-meshheading:16082727-Interferon-gamma, pubmed-meshheading:16082727-Interleukin-2, pubmed-meshheading:16082727-Mice, pubmed-meshheading:16082727-Mice, Inbred BALB C, pubmed-meshheading:16082727-Mice, Inbred C57BL, pubmed-meshheading:16082727-Mice, Knockout, pubmed-meshheading:16082727-Mice, Transgenic, pubmed-meshheading:16082727-Radiation Chimera, pubmed-meshheading:16082727-Receptors, Interleukin-2, pubmed-meshheading:16082727-Skin Transplantation, pubmed-meshheading:16082727-Specific Pathogen-Free Organisms
pubmed:year
2005
pubmed:articleTitle
Early regulation of CD8 T cell alloreactivity by CD4+CD25- T cells in recipients of anti-CD154 antibody and allogeneic BMT is followed by rapid peripheral deletion of donor-reactive CD8+ T cells, precluding a role for sustained regulation.
pubmed:affiliation
Bone Marrow Transplantation Section, Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural