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pubmed-article:16081995pubmed:abstractTextSimple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.lld:pubmed
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pubmed-article:16081995pubmed:monthAuglld:pubmed
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pubmed-article:16081995pubmed:authorpubmed-author:HongWanjinWlld:pubmed
pubmed-article:16081995pubmed:authorpubmed-author:BarkhamTimoth...lld:pubmed
pubmed-article:16081995pubmed:authorpubmed-author:InoueMasafumi...lld:pubmed
pubmed-article:16081995pubmed:authorpubmed-author:KeongLee...lld:pubmed
pubmed-article:16081995pubmed:authorpubmed-author:GeeLim SengLSlld:pubmed
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pubmed-article:16081995pubmed:volume43lld:pubmed
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pubmed-article:16081995pubmed:pagination4262-5lld:pubmed
pubmed-article:16081995pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:16081995pubmed:articleTitlePerformance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.lld:pubmed
pubmed-article:16081995pubmed:affiliationInstitute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sglld:pubmed
pubmed-article:16081995pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16081995pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed