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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2005-8-5
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pubmed:abstractText |
Simple gel-based one-step reverse transcription-PCR (RT-PCR) assays, used to investigate patients during the 2003 severe acute respiratory syndrome (SARS) outbreak in Singapore, were found to be as sensitive as commercial and in-house real-time RT-PCR assays. The detection limit was approximately 1 genome equivalent (GE) per 5 microl PCR mixture. One PFU of SARS coronavirus was estimated to be 258 +/- 46 GE.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-10871386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-12690091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-12730500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-12765977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-12765993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-12781537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15054079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15070991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15131154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15131160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15131168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15131175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15234807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-15488617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16081995-8783804
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4262-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2005
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pubmed:articleTitle |
Performance of single-step gel-based reverse transcription-PCR (RT-PCR) assays equivalent to that of real-time RT-PCR assays for detection of the severe acute respiratory syndrome-associated coronavirus.
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pubmed:affiliation |
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. mcbim@imcb.a-star.edu.sg
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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