Linked Life Data

16075244

Source:http://linkedlifedata.com/resource/pubmed/id/16075244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-9-21
pubmed:abstractText
Extracellular ATP is an important regulator of transepithelial transport in a number of tissues. In pancreatic ducts, we have shown that ATP modulates epithelial K+ channels via purinergic receptors, most likely the P2Y2 and P2Y4 receptors, but the identity of the involved K+ channels was not clear. In this study, we show by RT-PCR analysis that rat pancreatic ducts express Ca(2+)-activated K+ channels of intermediate conductance (IK) and big conductance (BK), but not small conductance (SK). Possible interactions between P2Y receptors and these Ca(2+)-activated K+ channels were examined in co-expression experiments in Xenopus laevis oocytes. K+ channel activity was measured electrophysiologically in oocytes stimulated with UTP (0.1 mM). UTP stimulation of oocytes expressing P2Y4 receptors and BK channels resulted in a 30% increase in the current through the expressed channels. In contrast, stimulation of P2Y2 receptors led to a 20% inhibition of co-expressed BK channel activity, a response that was sensitive to TEA. Furthermore, co-expression of IK channels with P2Y4 and P2Y2 receptors resulted in a large hyperpolarization and 22-fold and 5-fold activation of currents by UTP, respectively. Taken together, this study shows that there are different interactions between the subtypes of P2Y purinergic receptors and different Ca(2+)-activated K+ channels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
450
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
429-36
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16075244-Animals, pubmed-meshheading:16075244-Female, pubmed-meshheading:16075244-Intermediate-Conductance Calcium-Activated Potassium..., pubmed-meshheading:16075244-Large-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:16075244-Oocytes, pubmed-meshheading:16075244-Pancreatic Ducts, pubmed-meshheading:16075244-Potassium Channels, Calcium-Activated, pubmed-meshheading:16075244-Rats, pubmed-meshheading:16075244-Rats, Wistar, pubmed-meshheading:16075244-Receptors, Purinergic P2, pubmed-meshheading:16075244-Receptors, Purinergic P2Y2, pubmed-meshheading:16075244-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16075244-Small-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:16075244-Uridine Triphosphate, pubmed-meshheading:16075244-Xenopus laevis
pubmed:year
2005
pubmed:articleTitle
P2Y2 and P2Y4 receptors regulate pancreatic Ca(2+)-activated K+ channels differently.
pubmed:affiliation
Institute of Molecular Biology and Physiology, The August Krogh Building, University of Copenhagen, Universitetsparken 13, 2100, Copenhagen Ø, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't