Linked Life Data

16061948

Source:http://linkedlifedata.com/resource/pubmed/id/16061948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-9-19
pubmed:abstractText
Our objective was to evaluate the associations of individual apolipoprotein A-I (apoA-I)-containing HDL subpopulation levels with ABCA1- and scavenger receptor class B type I (SR-BI)-mediated cellular cholesterol efflux. HDL subpopulations were measured by nondenaturing two-dimensional gel electrophoresis from 105 male subjects selected with various levels of apoA-I in pre-beta-1, alpha-1, and alpha-3 HDL particles. ApoB-containing lipoprotein-depleted serum was incubated with [(3)H]cholesterol-labeled cells to measure efflux. The difference in efflux between control and ABCA1-upregulated J774 macrophages was taken as a measure of ABCA1-mediated efflux. SR-BI-mediated efflux was determined using cholesterol-labeled Fu5AH hepatoma cells. Fractional efflux values obtained from these two cell systems were correlated with the levels of individual HDL subpopulations. A multivariate analysis showed that two HDL subspecies correlated significantly with ABCA1-mediated efflux: small, lipid-poor pre-beta-1 particles (P=0.0022) and intermediate-sized alpha-2 particles (P=0.0477). With regard to SR-BI-mediated efflux, multivariate analysis revealed significant correlations with alpha-2 (P=0.0004), alpha-1 (P=0.0030), pre-beta-1 (P=0.0056), and alpha-3 (P=0.0127) HDL particles. These data demonstrate that the small, lipid-poor pre-beta-1 HDL has the strongest association with ABCA1-mediated cholesterol even in the presence of all other HDL subpopulations. Cholesterol efflux via the SR-BI pathway is associated with several HDL subpopulations with different apolipoprotein composition, lipid content, and size.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2246-53
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16061948-ATP-Binding Cassette Transporters, pubmed-meshheading:16061948-Adolescent, pubmed-meshheading:16061948-Adult, pubmed-meshheading:16061948-Aged, pubmed-meshheading:16061948-Animals, pubmed-meshheading:16061948-Apolipoprotein A-I, pubmed-meshheading:16061948-Carcinoma, Hepatocellular, pubmed-meshheading:16061948-Cell Line, pubmed-meshheading:16061948-Cell Line, Tumor, pubmed-meshheading:16061948-Cholesterol, pubmed-meshheading:16061948-Humans, pubmed-meshheading:16061948-Lipoproteins, HDL, pubmed-meshheading:16061948-Macrophages, pubmed-meshheading:16061948-Male, pubmed-meshheading:16061948-Mice, pubmed-meshheading:16061948-Middle Aged, pubmed-meshheading:16061948-Rats, pubmed-meshheading:16061948-Regression Analysis, pubmed-meshheading:16061948-Scavenger Receptors, Class B
pubmed:year
2005
pubmed:articleTitle
Differential effects of HDL subpopulations on cellular ABCA1- and SR-BI-mediated cholesterol efflux.
pubmed:affiliation
Lipid Metabolism Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA. bela.asztalos@tufts.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural